Sodium iodoacetate

Tryptic Digestion. The ozonized lysozyme solution after treatment with dithiothreitol in 9 M urea at pH 8.0 as described above, was carboxymethylated with sodium iodoacetate (0.02 M) at pH 4.6 and 25°C. for 24 hr, and purified by passage through a Sephadex G-25 column. Hydrolysis of lysozyme by trypsin (1%), and separation of the products were performed under the conditions as previously described by Jolles (12). 

Coupling of unsymmetrical dianions is also possible. Thus the acylsulfonamide dianion 7 couples with sodium iodoacetate to give 8 in high yield. On reduction and acidification, 8 is converted into the butyrolactone 9 in 75% yield.2... 

Methyl (E)-4-(r-butyldimethylsilyloxy)-3-methyl-2-butenoate, 187 Molybdenum carbonyl, 194 Pivaldehyde, 249 Potassium r-butoxide, 252 Sodium iodoacetate, 149 L-Valinol, 341 of enamines... 

From hydroxy acids or acyl derivatives Camphor-10-sulfonic acid, 62 Molybdenum carbonyl, 194 Sodium iodoacetate, 149 Thiophenol, 297... 

Antigen unmasking on sections of paraffin-embedded tissues can be accomplished by reduction of disulfide bonds by treatment with 2-mercaptoethanol, followed by alkylation with sodium iodoacetate to prevent the bonds from reforming. This method has been used for unmasking a Kunitz protease inhibitory domain epitope of Alzheimer s amyloid precursor protein in human brain (Campbell et al., 1999). Sections are reduced with a mixture of 0.14 M 2-mercaptoethanol in 0.5 M Tris-HCl (pH 8.0) and 1 mM EDTA for 3 hr in the dark at room temperature. After being washed for 3 min in distilled water, the sections are treated with a mixture of 250 mg/ml iodoacetic acid in 0.1 M NaOH, diluted 1 10 in 0.5 M Tris-HCl (pH 8.0) and 1 mM EDTA for 20 min in the dark. 

The carboxymethylation of cellulose with sodium chloroacetate in an aqueous system generally showed that the 2-OH group was more reactive than the 6-OH group, whereas the 3-OH was the least reactive. A low water content medium resulted in a relatively more uniform reactivity than a high water content reaction [202]. Similarly, carboxymethylation in a nonaqueous system (SO2-DEA-DMSO) [79] resulted in a more uniform reaction and a higher reactivity of the 6-OH group as compared to an aqueous reaction. Interestingly, carboxymethylation with sodium iodoacetate substantially enhanced the reactivity of the 3-OH, which was found to be most reactive under this condition. 

Conducted in reactions containing 7 and 14 mol water per mol cellulose, respectively. Conducted in SO, DEA-DMSO solution and alkali charge was percent cellulose. Conducted in SO,-DEA-DMSO using sodium iodoacetate. 

For laboratory measurements, an ISO method for pH (ISO 2917 1999) specifies either homogenization of the sample in potassium chloride solution and insertion of the electrode(s) into the extract, or introduction of the electrode into prepared holes in the nonhomogenized sample. Other methods specify preparation of a slurry of the ground sample with an equal weight of water, or preparation of a homogenate in the ratio of one part meat to five parts sodium iodoacetate (5mmoll )/potassium chloride (150mmol 1 ). 

In a commercial application of thiol formation by this nucleophilic substitution, the sodium salt of thioglycolic acid is prepared by the reaction of sodium hydrosuMde and sodium iodoacetate. 

The reduced, dialyzed carboxylase was carboxymethylated with sodium iodoacetate in the presence of guanidinium hydrochloride by standard procedures and then dialyzed exhaustively. Aliquots of the dialyzed samples, containing about 2 mg of protein, were hydrolyzed for amino acid analyses, and the remaining samples were digested with trypsin. 

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