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Sodium borohydride glutaraldehyde conjugation

Fig. 3. Immunofluorescence localization of tubulin in microtubules in Swiss 3T3 cultured fibroblasts. Swiss 3T3 cells were fixed in glutaraldehyde followed by treatment with sodium borohydride (4). Tubulin was detected using a rat monoclonal antitubulin antibody (YLl/2), followed by goat antirat IgG conjugated to rhodamine, all steps in the continuous presence of 0.1% saponin. Note the individual microtubules visible under the dark nuclear region (bar = 4 im). Fig. 3. Immunofluorescence localization of tubulin in microtubules in Swiss 3T3 cultured fibroblasts. Swiss 3T3 cells were fixed in glutaraldehyde followed by treatment with sodium borohydride (4). Tubulin was detected using a rat monoclonal antitubulin antibody (YLl/2), followed by goat antirat IgG conjugated to rhodamine, all steps in the continuous presence of 0.1% saponin. Note the individual microtubules visible under the dark nuclear region (bar = 4 im).
Preparation of FITC-Gelatin Coated Coverslips. Materials FITC-conjugated gelatin, 12-well glass coverslips, 12-well tissue culture plates, glutaraldehyde, PBS, sodium borohydride (Sigma, S-9125), and SF RPMI. [Pg.139]

See other pages where Sodium borohydride glutaraldehyde conjugation is mentioned: [Pg.779]    [Pg.473]    [Pg.311]    [Pg.132]    [Pg.453]    [Pg.304]    [Pg.304]    [Pg.108]    [Pg.304]    [Pg.324]   


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Glutaraldehyde

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