Skin graft sheets

Damage to skin can be caused by heat (burning), chemicals, electricity, ultraviolet or biting etc which results in 3-degree of skin trauma into epidermis, dermis and hypodermis respectively. As skin grafts, tissue engineering approach has evolved from simple cultured autologous epidermal sheets to more complex bilayered cutaneous substitutes (69). 

Uses Self-crosslinking emulsions, heat-curing coatings, silk grafting, crosslinked acrylic sheets, thermosetting paints Properties Pt-Co 100 max. colorless clear to yish. liq. faintly ester-like odor misc. with water m.w. 115.1 sp.gr. 1.1 b.p. 100 C solid, pt. -37 C flash pt. none pH 7 1 40 1.5% water Toxicology LD50 (oral, rat) 1815 mg/kg avoid contact with skin, eyes, mucous membranes TSCA listed... 

Autologous keratinocytes and fibroblasts have generally been derived from biopsies of the patient s uninjured skin [71 73]. Recently, keratinocytes have been isolated from the outer root sheath of plucked hair follicles [41,42]. The cells can be expanded in culture and used to populate skin substitutes in vitro. Grafted as epithelial sheets or as composites of biopolymers and cells, these are theoretically permanent skin substitutes once engraffment is achieved [25-27,33,41]. 

Three weeks after the skin was taken off, the patient was grafted with crrltttred keratinocyte sheet which had a 45 cm area in combination with the split-thickness autograft. 

Sheet autograft A layer of the patient s skin, comprising the epidermis and about one-third of the dermal thickness, that has not been meshed prior to grafting areas of severe skin loss. 

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