Size exclusion chromatography antibodies

Qian, R.L., Mhatre, R., and Krull, I.S., Characterization of antigen-antibody complexes by size-exclusion chromatography coupled with low-angle lightscattering photometry and viscometry, /. Chromatogr. A, 787, 101, 1997. 

Aurora Biomolecules dedicates to peptide synthesis (and polyclonal antibody production) for any small quantity purpose. FMOC chemistry (on Perceptive Biosystems Pioneer instruments) is used for peptides synthesis Online monitoring of the coupling efficiencies and HATU activation helps insure that the major component of the synthesis is the correct oligopeptide. Purification is firstly carried out by size exclusion chromatography, and then by HPLC on a PE vision purification workstation. Typically, 20 mg of pure peptide are obtained. The molecular weight of the purified peptide is determined as a final confirmation of quality. 

RG Nielsen, EC Rickard, PF Santa, DA Shakarnas, GS Sittampalam. Separation of antibody-antigen complexes by capillary zone electrophoresis, isoelectric focusing and high-performance size-exclusion chromatography. J Chromatogr 539 177-185, 1991. 

SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis HPLC, high performance liquid chromatography PCR, polymerase chain reaction IEF, isoelectric focusing HPSEC, high performance size-exclusion chromatography DNA, DNA-binding fluorochrome CPE, cytopathic effect Had, hemadsorption PMA, production of murine antibodies. 

Ammonium sulfate can easily be removed from the precipitated antibody preparations by dialysis of the solution against large volumes of the desired buffer. Gel filtration or size-exclusion chromatography is another method used for salt removal and buffer exchange. 

Alternatively, combined antibody fractions can be desalted using size exclusion chromatography columns such as PD-10 from GE Healthcare BioSciences. 

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