Site-selective immobilization

A unique feature of such DNA-directed self-assemblies is their site-selective immobilization, which makes it possible to construct well-defined nanostructures. On the other hand, the possibility of the introduction of a vast number of substitutes (like peptidic sequences, nucleoproteins, of hydrophobic hydrocarbon chains) to an adamantane core (adamantyl) makes such a process capable of designing steric colloidal and supramolecular conformations by setting hydrophobic/hydrophilic and other interactions. In addition, the rigidity of the adamantane structure can provide strength and rigidity to such self-assemblies [150]. 

Using this reaction fumaryl groups [24], cofactors [64] and carbohydrate derivatives [65] have been incorporated site selectively into folded four-helix bundle proteins. The reaction can also be expected to be of general use in site-selective immobilization reactions of folded proteins. 

M.S. Sanford, P.T. Charles, S.M. Commisso, J.C. Roberts and D.W. Conrad, Photoactivatable cross-linked polyacrylamide for the site-selective immobilization of antigens and antibodies, Chem. Mater., 10 (1998) 1510—1520. 

Petersen, S., Strohbach, A., Busch, R., et al. Site-selective immobilization of anti-CD34 antibodies to poly(L-lactide) for endovascular implant surfaces. J. Biomed. Mater. Res. B Appl. Biomater. 102(2), 345-355 (2013)... 

Niemeyer CM, Boldt L, Ceyhan B, Blohm D. DNA-Directed immobilization Efficient, reversible, and site-selective surface binding of proteins by means of covalent DNA-streptavidin conjugates. Anal Biochem 1999 268(1) 54—63. 

Watzke A, Kohn M, Gutierrez- Rodriguez M, Wacker R, Schroder H, Breinbauer R, et al. Site-selective protein immobilization by Staudinger Ugation. Angew. Chem. Int. Ed. Engl. 2006 45(9) 1408-1412. 

Noncovalent immobilization can also be used for I the site-selective coupling of antibodies to supports. One common approach for this involves absorbing the I antibody to a secondary ligand such as protein A or protein G, which both bind to the Fc region of many... 

Hodneland, C.D. et al.. Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands, Proc. Nat. Acad. Sci. USA, 99, 5048, 2002. 

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