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Solution denaturation

X Denhardt s solution 1% (w/v) Ficoll, 1% (w/v) polyvinyl pyrrolidone, 1% (w/v) BSA Filter-stenlize Store aliquots at -20°C. Do not flame the pipets used to transfer this solution. Denaturation and precipitation of the protein result from the use of hot pipets at this stage... [Pg.399]

Recently, considerable progress has been made on the calculation of electrostatic and hydrophobic interactions in biochemical systems 189 19 >. We can expect such calculations to become common for protein-solid surface interactions. Thus we can expect approximate values for the adsorption energy in selected systems to appear in the near future. The problem of time-dependent conformational adaptation of the protein to the surface (and vice versa) will be much more difficult. Initially, we will have to resort to crude measures of the structural stability of a protein, such as the temperature at which thermal denaturation occurs, the urea molar concentration for solution denaturation, etc. One or more of the models given in Fig. 13 should apply. [Pg.40]

Heat treatment of protein solutions denatures and precipitates some of the proteins, while others remain both soluble and stable. Thermal lability is determined empirically for each enzyme or protein of interest. [Pg.889]

Increasing tbe amounts of tbe organic solvent (metbanol or acetonitrile) and tbe acidic components of tbe solution denatures tbe protein, enabling tbe acquisition of extra charges. [Pg.175]

Modified Anson s Method Blood, which is generally considered to be a very difficult proteinaceous stain on fabrics, is used as substrate. A blood solution, denatured by urea. [Pg.654]

The conformability of protein and its susceptibility to denaturation is determined by its tertiary structure. The very structure of protein which determines its function also renders it susceptible to unfolding and precipitation from solution (denaturation). [Pg.46]


See other pages where Solution denaturation is mentioned: [Pg.371]    [Pg.576]    [Pg.292]    [Pg.87]    [Pg.64]    [Pg.78]    [Pg.115]    [Pg.212]    [Pg.235]    [Pg.226]    [Pg.292]    [Pg.183]    [Pg.302]    [Pg.467]    [Pg.1431]    [Pg.43]    [Pg.818]   
See also in sourсe #XX -- [ Pg.40 ]




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