Single-pass membrane proteins

The study of a conserved growth factor signaling pathway also led to intramembrane proteolysis. Epidermal growth factor (EGF) receptor ligands are synthesized as single-pass membrane proteins, but signaling requires the proteolytic release... 

Single-pass membrane proteins contain one or two topogenic sequences. In multipass membrane proteins, each ct-helical segment can function as an internal topogenic sequence, depending on its location in the polypeptide chain... 

The low sensitivity of both the static and MAS techniques of ssNMR spectroscopy has thus far limited the routine application of multidimensional experiments to determine the structure of membrane proteins in lipid bilayers. Mote et al. demonstrate the advantage of using a recently developed class of experiments, polarization optimized experiments, for substantial time-savings for 2D and 3D experiments. They demonstrate this methodology using sarcolipin, a single pass membrane protein. ... 

A special class of epithelial Na channels (ENaC), located in the apical epithelial membranes, mediate the active Na" " reabsorption in kidney, colon, lung, and sweat glands they are essential for the maintenance of the body s salt and water balance. The ENaC are completely different from the Nay channels and are not sensitive to TTX. Instead, they are inhibited with high affinity by amiloride, which is an established diuretic drug. ENaC form four channel subfamilies a, (3, y, and 5. Structurally they are 510 to 920 amino acid proteins with two TM segments separated by a large extracellular loop and have a single channel conductance of 4 to 5 pA. 

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