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Single-molecule imaging techniques image analysis

Whereas FCS measures fluorescence fluctuations over time, a related technique, ICS, measures fluorescence fluctuations over space, in particular from images collected using a laser-scanning microscope (56). ICS analysis of pixels within a single image provides information about protein clustering and density. A variation of ICS known as image cross-correlation spectroscopy evaluates the interactions of molecules labeled with different fluorescent probes. ICS can also be performed on stacks of... [Pg.204]

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Image analysis

Image-analysis techniques

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