Silver ion thin-layer chromatography

Ratnayake, W.M.N. Overview of Methods for the Determination of Trans Fatty Acids by Gas Chromatography, Silver-Ion Thin-Layer Chromatography, Silver-Ion Liquid Chromatography, and Gas Chromatography/Mass Spectrometry. J. AOAC International 87-. 523-539 (2004). 

Mossoba, M.E.R. Dugan, and J.K.G. Kramer. Methods for Analysis of Conjugated Linoleic Acids and trans- %-. Isomers in Dairy Fats by Using a Combination of Gas Chromatography, Silver-Ion Thin-layer Chromatography/Gas Chromatography, and Silver-Ion Liquid Chromatography, ]. AOAClnt.. 87, 545—562 (2004). 

Silver-ion chromatography can be a useful technique for isolation of a fraction enriched in CLA, but it can also afford unrivaled analytical separations. Silver-ion thin-layer chromatography (TLC) is best regarded as an enrichment method. With a mobile phase such as hexane/diethyl ether (9 1, vol/vol), CLA as the methyl ester elutes with cis-monoenes, rather than with a diene fraction with toluene as mobile phase, it elutes just ahead of the cw-monoenes (10). Alternatively, solid-phase extraction columns with bonded sulfonic acid moieties converted to the silver-ion form can be used with a simple elution scheme to obtain a combined monoene-CLA fraction (2,11). 

Nikolova-Damhanova, B. and Sv. Momchilova, Sy. Silver ion thin-layer chromatography of fatty acids A Survey, J. Liq. Chromatogr. Related TechnoL, 24,1447-1466, 2001. 

Nikolova-Darayanova, B., Chobanov, D., and Dimov, S. (1993). Separation of isomeric triacylglycerols by silver ion thin-layer chromatography. J. Liq. Chromatogr. 16 3997-4008. 

Nikolova-Damyanova, B., Christie, W. W., and Herslof, B. (1994). Improved separation of some positional isomers of monounsaturated fatty acids, as their phenacyl derivatives, by silver-ion thin layer chromatography. J. Planar Chromatogr.—Mod. TLC 7 382-385. 

Ratnayake, W.M.N., Overview of methods for the determination of trans fatty acids by gas chromatography, silver-ion thin-layer chromatography, and gas chromatography/mass spectrometry, J. AOAC Int., 87, 523-539, 2004. 

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