Signal Transduction Mechanisms in Sensors

In comparison to intensity-based methods, techniques that rely on FRET provide large changes in emission profiles and open the opportunity for ratio-metric fluorescence measurements [59]. Assays of this type are less prone to false positives from nonspecific binding events. 

In FRET-based assays, the light harvesting conjugated polymer and a flu-orophore capable of introduction into a probe structure are generally designed 

LUMO levels are nearly identical, as determined by electrochemical measurements. The PL quantum yields are also identical within experimental error. 

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