Sialic acids mass chromatography

Great progress in our knowledge about sialic acids was made with the aid of gas-liquid chromatography (g.l.c.), mass spectrometry (m.s.), and n.m.r. spectroscopy. Structural analysis of the many different N- andO-acylated Neu derivatives occurring in Nature will be described in Sections IV,5 and 6. 

Thus mass spectrometry showed the GPI was heterogeneous with at least five separate species and its composition was consistent with known GPI structures but with sialic acid as a novel component. The heterogeneity was confirmed by various separative techniques including capillary electrophoresis, Dionex high performance anion exchange chromatography (HPAEC), and reverse phase HPLC at pH 7. Cleavage... 

Gas-Liquid Chromatography and Mass Spectrometry of Sialic Acids... 

Klein, A., Diaz, S., Ferreira, L, Lamblin, G., Roussel, P., and Manzi, A.E. (1997) New sialic acids from biological sources identified by a comprehensive and sensitive approach liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of SILA quinox-alinones. Glycobiology (1997) 7, 421-432. 

Gamian, A., Romanowska, E., Dabrowski, U., and Dabrowski, J., 1991, Stmcture of the O-specific, sialic acid-containing polysaccharide chain and its linkage to the core region in lipo-polysaccharide from Hafnia alvei strain-2 as elucidated by chemical methods, gas-liquid chromatography/mass-spectrometry, and H NMR spectroscopy. Biochemistry 30 5032-5038. 

Kamerling, J. P., and Vliegenthart, J.F.G., 1982, Gas-liquid chromatography and mass spectrometry of sialic acids, in Sialic Acids (R. Schauer, ed.), Springer, New York, pp, 95-125. 

Kawai, T., Kato, A., Higashi, H., Kato, S., and Naiki, M., 1991, Quantitative determination of N-glycolylneuraminic acid expression in human cancerous tissues and avian lymphoma cell lines as a tumor-associated sialic acid by gas chromatography-mass spectrometry. Cancer Res. 51 1242-1246. 

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