Serum Analysis using Mid-infrared Spectroscopy

The two comprehensive feasibility studies have followed two different paths to avoid the problems associated with transmission spectroscopy of the native serum. One of 

1 Attenuated Total Refleaonce Spectroscopy of Native Serum 

In this work the investigators sought to quantify glucose, total protein, cholesterol, triglycerides, urea, and uric acid on the basis of the MIR ATR spectra collected using a CIRCLE ATR cell (Spectra-Tech Inc., Shelton, CT, USA). It had been concluded on the basis of 

When a small volume of serum is spread evenly on an IR-transparent window and allowed to dry, the resulting film may be used as the basis to quantitate at least six analytes. A large study based upon this approach used 200 specimens as the basis to develop PLS calibration models for glucose, triglycerides, total protein, albumin, cholesterol, and urea, and an additional 100 specimens to test the accuracy of these models.  

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R.A. Shaw, S. Kotowich, M. Leroux, H.H. Mantsch, Multianalyte Serum Analysis using Mid-infrared Spectroscopy , Ann. Clin. Biochem., 35, 624-632 (1998). 

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