SERS-Based Biosensors

Biosensors Using Surface-Enhanced Raman Scattering, Fig. 4 Layout of a gold array-embedded gradient chip for the SERS-based immunoassay. The illustrations in the enlarged circles represent the formation of sandwich... 

Due to the molecular specificity of SERS/SERRS, combined with its inherent sensitivity, it has found utility in a wide range of applications, only a few of which can be described here. The development of SERS sensor technology has allowed the detection and identification of seven structurally similar monosaccharides in aqueous solution using a sample volume of only 5 pi with a concentration of 1x10 mol dm This has led to the initial development of a glucose-based biosensor with true in vivo, real time, minimally invasive sensing. 

Cancer diagnostics often needs a discrimination of genomic mutations in DNA sequences or special NA structures (miRNA) which can cause various diseases including cancer. SERS technique is very promising for mutation and single nucleotide polymorphisms detection because of the possibility of spatial multiplexing at array format linked with a portable biosensor device. Mahajan and co-workers reported such SERS-based approach using Au substrates prepared by... 

The unique photonic and condnctive properties of some nanoparticles can be employed to detect biological recognitions on a surface. With dimensions similar to those of biomolecnles, nanoparlicles are a natural choice for detecting biomolecules, which can be nsed in both electrochemically and optically based biosensors. Upon the assembly of nanoparticles on a solid surface, the biomolecules adsorbed on the surfaces of nanoparlicles can be detected by means of surface plasmon resonance (SPR), surface enhanced Raman spectroscopy (SERS), and surface-enhanced fluorescence spectroscopic techniques. Furthermore, the unique size-controlled optical properties of semiconductor nanoparticles imply that the organization of combinatorial hbraries of biomolecule-semiconductor nanoparticle hybrid systems or the assembly of these hybrids in array configurations may lead to the high-throughput parallel analysis of numerous analytes [53]. 

Pu KY, Liu B (2010) Fluorescence reporting based on FRET between conjugated polyelectrolyte and organic dye for biosensor applications. In Demchenko AP (ed) Advanced fluorescence reporters in chemistry and biology. II. Springer Ser Fluoresc 8 417 -53... 

The range of sample characteristics and manner of their detection, is much larger than can be realistically addressed in the space of a single chapter. We will confine this chapter mainly to the chemical sensor research areas discussed in other chapters in this volume, dividing them into electrical, optical, and mass and thermal measurements. Our focus will furthermore be on the generic chemical and physical phenomena upon which such measurements can be based, as opposed to the alternative organization that would address chemical sensors in the context of their application (i.e, auto exhaust sensor, clinical diagnostic sensor, environmental sensor) or of the kinds of samples detected (i.e, CO sensors, humidity sensor, biosensor, etc.), as used in a previous ACS Symposium Series volume on Chemical Sensors (D. Schuetzle, R. Hammerle, Eds., ACS Sympos. Ser. 309, 1986). 

C. Song, et al.. Highly sensitive immunoassay based on Raman reporter-labeled immu-no-Au aggregates and SERS-active immune substrate, Biosensors and Bioelectronics 25 (4) (2009) 826-831. 

Li, F., Wang, Z., and Feng, Y. (2009) Construction of bienzyme biosensors based on combination of the one-step electrodeposition and covalent-coupled sol-gel process. Sci. China Ser. B, 52, 2269-2274. 

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