Sequencing/sequences promoter sequence

Differences in receptor distribution are due to species differences in region-specific gene expression. Across vole species, OT receptor coding sequences and promoter sequences are virtually identical species diversity in receptor expression appears to relate to quantitative and regional differences rather than qualitative aspects of the receptor when expressed (Insel et al., 1997). 

EMBL (European Molecular Biology Laboratory) [33] is a nucleotide sequence database provided from the online host EBl. Release 73 (December, 2002) consists of over 20 million nucleotide sequences with more than 28 billion nucleotides. The information includes sequence name, species, sequence length, promoter, taxonomy, and nucleic acid sequence. 

FIGURE 28 10 During transcription a molecule of mRNA is assembled from a DNA template Transcription begins at a promoter sequence and proceeds in the 5 3 direction of the mRNA until a termination sequence of the DNA is reached Only a region of about 10 base pairs is unwound at any time... 

If an antibody to the protein of interest is available, it is sometimes possible to use vector sequences, eg, the beta-galactosidase promoter sequence, to direct the transcription of the passenger DNA into messenger RNA and the translation of that mRNA into protein which can be recognized by the antibody. Although this method is somewhat less reHable than the use of nucleic acid probes, specialized vectors are available for this purpose. 

Ellis, J.G., Llewellyn, D.J., Dennis, E.S. Peacock, W.J. (1987). Maize Adh-1 promoter sequences control anaerobic regulation addition of upstream promoter elements from constitutive genes is necessary for expression in tobacco. EM BO Journal, 6, 11-16. 

The -10 region Indicates the second half of the potential promoter sequence recognized by the vegetative sigma factor. 

CaMV 35S promoter a promoter sequence from the CaMV... 

Uchino, K., Imamura, M., Sezutsu, H. et al. 2006. Evaluating promoter sequences for trapping an enhancer activity in the silkworm Bombyx mori. J. Insect Biotechnol. Sericology, 75 89-97. 

Min B, Augustin L, Felsheim R, Fuchs J, Loh HH. Genomic structure and analysis of promoter sequence of a mouse mu opioid receptor gene. Proc Natl Acad Sci USA 1994 91 9081-9085. 

The first step in creating a specific mRNA species is to construct a plasmid encoding the mRNA behind a T7, T3, or SP6 RNA polymerase promoter sequence. The S boundary of the mRNA is defined by the position of the promoter transcription start site and the S end is defined by restriction digest of the plasmid prior to in vitro transcription. 

Increased transcription levels are assumed to result in increased protein synthesis. One approach to reach this goal is to raise the transgene copy number by the use of amplification-promoting sequences derived from a spacer sequence of tobacco ribo-somal DNA [95]. Posttranscriptional processes such as capping, splicing and polya-denylation are important for high protein yields, and it is also important to maximize mRNA stability [84]. 

Potaman, V. N., Ussery, D. W. and Sinden, R. R. Formation of a combined H-DNA/open TATA box structure in the promoter sequence of the human Na,K-ATPase alpha2 gene. J. Biol. Chem. 271 13441-13447,1996. 

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