Sequencing conformation polymorphism analysis

Single-strand conformation polymorphism analysis (SSCP) Simple Short sequences only (<200bp) does not reveal position of change... 

Other methods that did not require sequence information but produced species-specific electrophoretic gel patterns were also developed. Single-strand conformational polymorphism analysis is one such method, which was used to discriminate fish and meat products. In this method, the same region of the DNA is amplified, double-stranded amplification products are denatured and single-stranded products are allowed to form their sequence-dependent secondary structures, and their corresponding migration patterns on the gel are observed [51]. 

Saed G, Ladin D, Olson J (1998) Analysis of p53 gene mutations in keloids using polymerase chain reaction-based single-strand conformational polymorphism and DNA sequencing. Arch Dermatol 134 963-967... 

SSCP) This typing method is based on conformation polymorphism of single strands derived from polymorphic sequences. In S. aureus, the method was used for analysis of DNA gyrase A subunit gene (gyrA) (Tokue et al. 1994). This method has been used successfully for molecular identification of bacterial species based on 16S rRNA gene (Widjojoatmodjo et al. 1995 Delbes and Montel 2005). 

One of the most popular methods for identifying differences in the sequence of ampUcons is single-stranded conformation polymorphism (SSCP) analysis, which has been used very often in chnical genetic research (Hayashi, 1992 Bunge et al., 1996 Hestekin and Barron, 2006). In January 2007 more than 10,000 publications were listed in PubMedunder the term SSCP. In the last two decades, SSCP has also been found useful in tackling various problems related to food analysis. 

DNA and RNA with different sequences and mutations show different electrophoretic migration behavior. There are simple electrophoretic methods, which can be used to detect mutations and minimal differences within the sequences of the PCR product. Popular methods are single-strand conformational polymorphism and heteroduplex analysis. 

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