Separatory and Analytical Methods

Methods for the quantitative chromatographic analysis of inositol phosphates and inositol phospholipids have been reviewed.  

The chromatography of carbohydrates and related compounds has been re-viewed.  

separation of various methylated derivatives of mannose, galactose, and glucosamine as their trimethylsilylated derivatives has been described using 0.3-0.4% OV 225 on Chromosorb that had been surface modified by a high molecular weight polyethylene glycol. Conditions have been found for the separation of the TMS ethers of arabinose, xylose, mannose, ot- and /3-glucose, fructose, sorbose, xylitol, mannitol, and sorbitol on Chromosorb W-NAW with 15% Carbowax 20M, and a g.l.c. method for the quantitative determination of trehalose as its TMS ether has been reported.  

The apparent relative decline in this chapter cannot be taken as evidence that separations and analyses are becoming less significant in carbohydrate chemistry. Rather the reverse seems more probable many sophisticated methods are being used routinely, their availability is taken for granted and they are not identified as key features of papers.  

Gas-Llquld Chromatography.- There have been several reports on the separation of enantiomeric sugars using capillary g.l.c. Diastereo-isomeric dithioacetals, from seven aldoses with (+)-l-phenylethane-thiol-trifluoroacetic acid, were separated as their peracetylated or pertrimethylsilylated derivatives. Trifluoroacetylated 0-(-)- 

A useful strategy for rapidly acquiring a library of reference data in conjuction with methylation analysis of polysaccharides 

Including a method for obtaining reproducible retention coefficients using two internal standards, and another paper describes over 242 such derivatives from eight alditols. The suitability of a variety of stationary phases for capillary separation of alditol acetates and their partially methylated analogues derived from neutral and amino-sugars has been investigated, while the separation of such compounds on packed columns has also been 

N-deacetylation, nitrous acid deamination, and reduction of sialic 

The preparation of peracetylated aldononitriles from neutral and aminosugars using N-methylimldazole as catalyst and solvent has been further detailed. 

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Separatory and Analytical Methods Synthesis of Enantiomerically Pure Non-carbohydrate 53 96 77 122 23 ... 

Separatory and Analytical Methods Chromatographic Methods Gas-Liquid Chromatography Thin-layer Chromatography High-pressure Liquid Chromatography Column Chromatography Electrophoresis Other Analytical Methods... 

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