Separator Shim-pack

Sultana et al. [88] developed a reversed-phase HPLC method for the simultaneous determination of omeprazole in Risek capsules. Omeprazole and the internal standard, diazepam, were separated by Shim-pack CLC-ODS (0.4 x 25 cm, 5 m) column. The mobile phase was methanol-water (80 20), pumped isocratically at ambient temperature. Analysis was run at a flow-rate of 1 ml/min at a detection wavelength of 302 nm. The method was specific and sensitive with a detection limit of 3.5 ng/ml at a signal-to-noise ratio of 4 1. The limit of quantification was set at 6.25 ng/ml. The calibration curve was linear over a concentration range of 6.25—1280 ng/ml. Precision and accuracy, demonstrated by within-day, between-day assay, and interoperator assays were lower than 10%. 

Cetin and Atmaca [41] reported HPLC analysis of vigabatrin in tablets by precolumn derivatization with l,2-naphthoquinone-4-sulfonic acid sodium salt and detected at 451 nm. Separation was achieved on a Shim-Pack CLS-ODS (5 /im) column (25 cm x 4 mm) with a mixture of 10 mM phosphoric acid-acetonitrile (75 25) as a mobile phase (flow rate... 

The major constituents of the plant Ambrosia maritima L. were isolated using TLC and identified by IR and GC-MS. From HPLC screening, ambrosin and damsin were detected at high levels in the leaves and flowers, while stems and seeds contained only traces. Sesquiterpenes from Aucklandia lappa (Decne.) were determined by HPLC. A Shim-pack CLC-ODS column (6.0 x 150 mm) was used as separation column, Me0H-H20 (65 35) as mobile phase, and the detection wavelength was 225 nm. 

In a typical experiment, 35 ml of aqueous solution into which both CO2 and NH3 or ammonium carbonate had been dissolved (pH = 8.9) was sealed in a quartz ampule and irradiated at 90 °C for 100 hr. The resulting solution was heated at 90 °C to dryness under the reduced pressure in order to remove the remaining NH3 or ammonium carbonate and the residue (white) was hydrolyzed at 105 °C for 48 hr in 6 M HCl. After evaporating the HCl solution at 90 °C to dryness, the residue was dissolved with 10 ml of water. Amino acids in the solution were separated by HPLC (SCL-6A, product of Shimadzu Co.) equipped with a column of cation exchange resin (Shim-pack 1SC-07/S1504L1, a product of Shimadzu Co.) and detected based on the ninhydrin reaction [13]. Nucleic acid bases and organic acids such as oxalic and oxamic acids were separated by HPLC with an CDS reversed phase column (Shim-pack CLC-ODS) and detected spectrophotometrically. 

Figure 16 Effects of pH and salt type on band spacing in the anion-exchange separation of a protein sample 1, myoglobin 2, transferrin 3, a,-acid glycoprotein 4, a-lactalbumin 5, soybean trypsin inhibitor. Conditions 5 x 0.4-cm WAX column (300A pore) (Shimadzee Shim-pack) 0-100% B in 20min I.OmL/min. (a) A solvent, 0.02 M phosphate buffer B solvent, 0.5 M phosphate buffer (b) A solvent, 0.02 M phosphate buffer B solvent 0.5 M NaCI or Na2S04. (From Ref. 41.)...

Shim-pack SCR-102H Shimadzu 300 x8 7 Sulfonate groups High-performance separator for weak organic acids... 

Figure 10.208 Ion-exclusion chromatography eluent 5mmol/L H2SO4 detection UV of organic acids in a freshly squeezed grape (210nm) sample freshly squeezed Italian juice utilizing UV detection. Separator column grape juice peaks (1) tartrate, (2) malate, and Shim-pack IE column temperature 60°C (3) fumarate (see [311]).

Circular strip dividers are located between each strip (Fig. 14). The dividers are used to separate and align the strands. Typically, two and, in some cases, three divider assemblies are used throughout the line. Each divider assembly must be set up individually. Similar to the packed arbor slitter, spacers and shims are... 

---