Separator CarboPac

In high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) system, anion exchange column is used for CD separation. CarboPac PA-10 and 100 are all selected for CD qualitative analysis. The main eluent for the column of CarboPac PA-100 is sodium hydroxide, the gradient eluent is sodium acetate. 

Separator CarboPac CarboPac CarboPac CarboPac CarboPac CarboPac CarboPac Metrosep Metrosep... 

Oligalacturonides were separated on a Dionex Bio LC with a CarboPac PA 1 column and pulsed amperometric detection using a linear Na-acetate-gradient in 0.1 M NaOH. 

Alkaline conditions are used so frequently in carbohydrate separations on CarboPac columns that it should be pointed out that acidic conditions are suitable for separation of acidic sugars. Figure 24 shows the separation of sialic acid containing oligosaccharides.256 Alkaline conditions were used for neutral milk oligosaccharides and mucin oligosaccharide alditols were characterized similarly.257 The carbohydrates released from yeast mannopro-tein with N-acetyl-P-D-glucosaminidase were also fractionated on CarboPac ... 

Two anion exchange columns Dionex CarboPac PA1 plus a guard column and CarboPac MAI column with a guard column were used for separation procedure (High Performance Anion Exchange Chromatography). 

The product, 2-keto-3-deoxy-6-phosphogluconate, was separated by chromatography at room temperature and a flow rate of 1 mL/min on a Dionex CarboPac PA-1 column (4 mm x 250 mm). The mobile phase was composed of 24 mM NaOH and 300 mM sodium acetate for 5 minutes, followed by a linear gradient to 700 mM sodium acetate in 10 minutes. A linear gradient back to the initial conditions was run in 5 minutes. Pulsed amperometric detection was used with a pulse train consisting of a 480 ms detection pulse at +80 mV, followed by pulses of 120 ms at +600 mV and 60 ms at -600 mV. 

Table 1 Separation of Carbohydrates, Alditols, Alcohols, and Glycols using a CarboPac MAI Column and Pulsed Amperometry...

Fig. 3-50. Separation of sulfide and cyanide in a strongly alkaline solution. — Separator column IonPac AS6 (CarboPac) eluent 0.1 mol/L NaOH + 0.5 mol/L NaOAc + 0.0075 mol/L ethylenediamine flow rate 1 mL/min other chromatographic conditions see Fig. 3-49.

Fig. 3-56. Separation of fluoride, iodate, bromate, and chloride. - Separator column IonPac AS6 (CarboPac) eluent 0.0034 mol/L NaHCO, + 0.0036 mol/L Na2C03 flow rate 1 mL/min detection suppressed conductivity injection volume 50 pL solute concentrations 2 ppm fluoride, 10 ppm iodate, 20 ppm bromate, and 5 ppm chloride.

Fig. 3-84. Separation of various monocarboxylic acids at an anion exchanger IonPac AS6 (CarboPac). - Eluent 0.0017 mol/L NaHCOj + 0.0018 mol/L Na2C03 flow rate 1 mL/min detection suppressed conductivity injection 50 pL solute concentrations 3 ppm fluoride (1), 40 ppm acetic acid (2), 20 ppm glycolic acid (3), 10 ppm a-hydroxyisocaproic acid (4), 20 ppm formic acid (5), oxamic acid (6), methanesulfonic acid (7), amidosulfonic acid (8), and a-ketoisocaproic acid (9).

Fig. 3-105. Separation of various sugar alcohols and saccharides. - Separator column CarboPac PA-1 eluent 0.15 mol/L NaOH flow rate 1 mL/min detection pulsed amperometry at a Au working electrode injection volume 50 pL solute concentrations 10 ppm xylitol, 5 ppm sorbitol, 20 ppm each of rhamnose, arabinose, glucose, fructose, and lactose, 100 ppm sucrose and raffmose, 50 ppm maltose.

Fig. 106. Dependence of the retention of some selected carbohydrates on the hydroxide ion concentration. - Separator column CarboPac PA-1 flow rate 1 mL/min detection and injection volume see Fig. 3-105 solute concentrations 100 ppm each of the respective carbohydrates.

Fig. 3-113. Separation of maltose oligomers. - Separator column CarboPac PA-1 eluent (A) 0.1 mol/L NaOH, (B) 0.1 mol/L NaOH + 1 mol/L NaOAc gradient 100% A for 2 min isocratically, then linearly to 100% B in 200 min flow rate 1 mL/min detection and injection volume see Fig. 3-105 solute concentrations 166 ppm each of maltose (1), maltotriose (2), maltotetraose (3), maltopentaose (4), maltohexaose (5), and maltoheptaose (6).

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