Separation time high-speed

It is seen from Figure 15 that the analysis time ranges from about 10,000 seconds (a little less than 3 hr) to about 30 milliseconds. The latter, high speed separation, is achieved on a column about 2 mm long, 12 microns in diameter, operated at a gas velocity of about 800 cm/second. Such speed of elution for a multicomponent mixture is of the same order as that of a scanning mass spectrometer. 

Plate valves are typically used for high speed separable compressors. Plate valves are similar to ring valves with the rings connected by ribs. Instead of individual elements opening and closing, all valve ports o[)en and close at the same time. 

At high speeds, however, a position can be reached in which the load-carrying capacity increases with speed. The explanation is thought to be that when the teeth are in contact for such a short time, the oil film is not squeezed out and a separating film is therefore maintained. 

A high-speed separation of tryptic peptides of horse cytochrome c is shown in Figure 6. As the temperature and flow rate were increased, the separation substantially improved, an observation consistent with data demonstrating that conformational issues may be increasingly important factors in peak broadening as the time scale of the chromatography approaches that... 

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