Separation of Lipids According to Compound Class

Thin-Layer Chromatography of Lipids 1. Separation of Lipids According to Compound Class 

Over 25 years ago, Trappe showed that fractionation of lipids into compound classes was possible by elution from adsorbent columns using a succession of solvents of varying polarity. He classified solvents of differing polarities in the order of their elution effect and named this list the eluotropic series of solvents . Trappe s eluotropic series and further data on solvents are quoted on p. 202. 

Several authors have found that mixtures of petroleum ether and diethyl ether in various proportions could be used to advantage instead of a series of individual solvents. Procedures for chromatographic 

After Stahl hadintroducedTLC [193], several authors [69,78,117,214] apphed this new method to lipids and found that it was splendidly suitable for separating compounds in this group of natural products. It is evident from a recently published review [152] that thin-layer and gas chromatography have almost completely displaced other chromatographic procedures for lipid separation within a few years. 

Pubhcations on the TLC of lipids which had appeared up to spring 1964 have been listed in two compilations [120, 122]. 

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