Sensitivity analysis, clinical economic

Recently, CE coupled with Ru(bpy)3 ECL for highly sensitive detection of metformin hydrochloride (MH) derivatized with acetaldehyde is reported. The precolumn derivatization of MH with acetaldehyde was performed in phosphate buffer solution (0.3 mol L pH 7.5) at room temperature for 120 min. The factors affecting this method of analysis, e.g., acetaldehyde concentration, buffer pH, electrokinetic voltage and injection time were examined and under optimization of these conditions, the MH ECL detection sensitivity was more than 120 times that without derivatization. The detection of 0.3 ng mL with S/N = 3 was attained. The proposed method is simple, economical, and sensitive and is used for the determination of MH in urine [42]. A method combining CE with Ru(bpy)3 " ECL detection that can be applied to amine-containing clinical species was developed, and the performance of CE-ECL as a quantitative method for the determination of sulpiride in human plasma or urine was evaluated [43]. 

Unlike other tests, the eNO test is fast, easy to perform, economical and presents essentially no risk to the patient. Also, it provides an assessment of underlying airway inflammation, which is a chronic condition in asthma patients. Therefore, the specific TOLAS set-up used by Roller and co-workers, with its ability to determine eNO concentrations in real time with parts per billion sensitivities, may prove to become a capable and user-friendly tool for next-generation eNO analysis to diagnose and monitor asthma in clinical settings. 

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