Sensing process/protocols

Furthermore, the development of electronic transduction means that probe the DNA sensing events on the transducers is essential. It is also desirable to develop sensing protocols that could later adapt a chip configuration that enables the screening of multiple sensing processes. 

The Nozaki protocol, - which is an organoaluminum-ate mediated 1,4-addition followed by a 1,2-addition of the resulting enolate, may be considered a 1-acylethenyl anion equivalent, which does fall within the scope of this review. This process may be conducted in either an intramolecular sense or an intermolecular sense. In general, the protocol is compatible with a wide array of functionality however, the intermolecular process requires an aldehyde, whereas the intramolecular case may be terminated by either an aldehyde or a ketone. 

If protocol development is rate determining, the effectiveness of ADME experimental assays depends very much on how the early exemplars are synthesized. In theory, the most effective method would be to obtain a well-spaced subset of the library in an experimental design sense. A traditional, noncombinatorial, synthesis would accomplish this but would not fit in well with a combinatorial optimization process. A possible means around this problem is to institute some type of early automated clean-up of combinatorial exemplars from partially optimized reaction schemes. This is not a tidy solution because the most efficient process would be an automated clean-up on the entire library after the optimization process was complete. 

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