Selective export

ER-Associated Degradation, when proteins mis-fold in the ER due to mutation or environmental conditions, they are selectively exported to the cytosol for degradation by the proteasome. 

Expoitins are transport receptors at the nuclear pore complex needed for the selective export of proteins from the nucleus into the cytoplasm. They recognize nuclear export signal sequences of cargo proteins. 

Select the Library (libref) and Member (data set) to be exported, and then click Next to open the Select Export Type window. To get a CSV file, select Comma Separated Values as the standard data source, as follows ... 

There are other options for creating delimited ASCII files from the Export Wizard. From the Select Export Type window you can pick tab delimited or delimited file as one of the standard data sources if you want delimited files. If you choose the delimited file option, you can choose your field delimiter by clicking the Options button. For instance, if you were creating a pipe-delimited file, the Select Export Type window would look like this ... 

You can see how the (pipe) character is entered as the delimiter in the Options dialog box. If you have more complex requirements for the ASCII text file you want to export, you can invoke External File Interface in the Select Export Type window, write customized DATA step code with FILE and PUT statements, or use some of the ODS tagsets supplied by SAS, found at http //support.sas.com/md/base/topics/odsmarkup/, that have the ability to create numerous types of ASCII text formats. 

Another approach to exporting text files with SAS is to use the SAS Enterprise Guide interface. The following example exports the DM data set to a CSV file using SAS Enterprise Guide 3.0. With the DM data set selected in the Process Flow window, click File and then select Export dm from the drop-down list as follows ... 

Nishimura, N., and Balch, W. (1997). A di-acidic signal required for selective export from the endoplasmic reticulum. Science. 277, 556-558. 

Roberg, K.J., Crotwell, M., Espenshade, P., Gimeno, R., and Kaiser, C. A. (1999). LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum./. Cell Biol. 145, 659-672. 

One property of the translocase is specially noteworthy its affinity for long-chain acylcamitine is very much higher than for carnitine itself. This suggests a basic role for carnitine, additional to the acetylation buffer already mentioned. Every fourth reaction of the P-oxidation spiral (the excision of an acetyl unit by a thiolase enz5mie) requires free CoASH, so that if the CoA of the mitochondrial matrix became over-acy-lated the whole process would come to a halt. If acyl-CoA were formed directly from fatty acids in the same compartment (the matrix) as oxidation this might readily happen. However, the carnitine system guards against this fail-nonsafe situation, because if the acyl-CoA/CoASH ratio (and hence the acylcamitine/camitine ratio) rises the translocase will selectively export acylcamitine from the matrix and import carnitine this will lower the ratio and restore P-oxidation. It remains (I think ) to be seen if this effect can be conclusively demonstrated. 

The procedure for exporting is to click on File at the top left comer of the Aspen Plus window and select Export. The window shown in Figure 7.5 opens and the drop-down menu is used to select P-driven Dyn Simulation, which is the 12th item on the list. Then the Save button is clicked. Make sure you know the location where you are saving the file. 

Aspen Plus allows you to generate a report file containing the simulation inputs and calculated results. From the Aspen Plus menu bar, select File and then select Export. The export... 

We are now ready to export the file. Go to File and select Export. The window shown in Figure 4.21 appears on which a P Driven Dyn Simulation (. dynf, dyn.appdf) is selected and the Save button is clicked. A message window appears, as shown in Figure 4.22, with some information about the need to install a hquid level controller and the use of a performance curve in the pump. No fatal errors are reported, so the export has been successful. 

To reverse this process and extract the original media files from an. acd-zip file, from the File menu, select Export Loops. Browse for a location to save the fdes to and click the Save button. It is not a bad idea to create a new folder to store these files in, because there can sometimes be quite a few files in a project. You can select a format for the new media files finm the Save as type list (for example,. wav). Selecting a compressed format such as. mp3 can save room on your disk, but the quality of the loops may suffer. See Chapter 10 on Publishing for more information on file formats, compression, and quality. 

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