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Selection Marker hygromycin

Although not as popular as the baculovirus-system, stable transformations of insect cells can be used to circumvent the problems mentioned in the last paragraph. Common hosts include the fruitfly and mosquito. The expressed genes are often under the control of the Drosophila metallothionein promoter. Genes coding for resistance to antibiotics such as hygromycin and neomycin are used as selection markers. [Pg.295]

The second component of an expression vector comprises DNA sequences required for the selection, maintenance and copy-number amplification of the vector in the host cell wherein protein expression is desired. Obviously, this region is not needed for expression in E. coli. The selectable markers used in other host cells may be an enzyme conferring the ability to produce a nutrient essential for growth of the host cell, or transformation of cell morphology, or resistance to antibiotics (such as hygromycin or geneticin). Sequences for the maintenance of the vector DNA are required for the autonomous replication of the plasmid in these host cells except in cases where the vector sequences Tntegrate into chromosomes of the... [Pg.49]

Nakamura, A., Takakura, Y., Kobayashi, H., and Hoshino, T. (2005) In vivo directed evolution for thermostabilization of Escherichia coli hygromycin B phosphotransferase and the use of the gene as a selection marker in the host-vector system of Ihermus thermophilus. J. Biosci. Bioertg., 100, 158-163. [Pg.566]

Cannio, R. Contursi, P. Rossi, M. Bartolucci, S. Thermoadaptation of a mesophilic hygromycin B phosphotransferase by directed evolution in hy-perthermophilic Archaea selection of a stable genetic marker for DNA transfer into Sulfolobus solfataricus. Extremophiles, 5, 153-159 (2001)... [Pg.59]

Hygromycin B [29] is an alternative antibiotic resistance marker for the selection... [Pg.665]

See other pages where Selection Marker hygromycin is mentioned: [Pg.252]    [Pg.45]    [Pg.59]    [Pg.596]    [Pg.17]    [Pg.18]    [Pg.154]    [Pg.252]    [Pg.1427]    [Pg.653]    [Pg.771]    [Pg.109]    [Pg.1367]    [Pg.346]    [Pg.608]    [Pg.699]    [Pg.132]    [Pg.119]    [Pg.119]    [Pg.51]    [Pg.53]    [Pg.54]    [Pg.385]    [Pg.401]    [Pg.5]    [Pg.62]    [Pg.734]    [Pg.766]    [Pg.264]    [Pg.142]    [Pg.384]   
See also in sourсe #XX -- [ Pg.62 ]



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