Selecting a Stationary Phase for an Application

Once a reasonably suitable column phase has been identified, the dimensions of the column employing it should be selected, and then the separation is optimized by varying the flow and temperature parameters. The column dimension decisions will depend on the difficulty of achieving the necessary peak resolutions, which is likely to increase with the number of analytes to be measured. The column s analyte capacity depends on these dimensions as well. The column dimensions, carrier gas flow, and column oven temperature parameters thus chosen wiU determine the time required to perform each GC run. 

Sample focused at column front and starts to migrate normally 

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