Segmental isotopic labeling

Yamazaki T, Otomo T, Oda N, Kyogoku Y, Uegaki K, Ito N, Ishino Y, Nakamura H, Segmental isotope labelling for protein NMR using peptide splicing, J. Am. Chem. Soc., 120 5591-5592, 1998. 

Otomo T, Teruya K, Uegaki K, Yamazaki T, Kyogoku Y, Improved segmental isotope labeling of proteins and application to a larger protein, J. Biomol. NMR, 14 105-114, 1999. 

Xu R, Ayers B, Cowbum D, Muir TW, Chemical ligation of folded recombinant proteins Segmental isotopic labelling of domains for NMR studies, Proc. Natl. Acad. Sci. USA, 96 388-393, 1999. 

Blaschke, U. K., Cotton, G. J., and Muir, T. W. (2000) Synthesis of multi-domain proteins using expressed protein hgation Strategies for segmental isotopic labeling of internal regions. Tetrahedron 56, 9461-9470. 

Otomo T, Ito N, Kyogoku Y, Yamazaki T. NMR observation of selected segments in a larger protein central-segment isotope labeling through intein-mediated ligation. Biochemistry 1999 38 16040-16044. 

Romanelli A, Shekhtman A, Cowburn D, Muir TW. Semisynthesis 46. of a segmental isotopically labeled protein splicing precursor ... 

Cowburn D, Muir TW. Segmental isotopic labeling using expressed protein ligation. Meth. Enzymol. 2001 339 41-54. 48. 

Segmental isotopic labeling has been accomplished using both protein transsplicing and EPL. Yamazaki and coworkers used a protein transsplicing... 

T. W. Muir, Autoregulation of a bacterial a factor explored by using segmental isotopic labeling and NMR, Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 8536-8541. 

Segmental Isotopic Labeling Prospects for a New Tool to Study the Structure-Function Relationships in Multi-Domain Proteins... 

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