Scattering intrinsic fluorescence

The disadvantage of the nitrocellulose (NC) slide relates to resolution because biomolecules more easily diffuse from the surface than with planar arrays. Also, membranes suffer from considerable light scatter and higher intrinsic fluorescence, which are problematic for increased sensitivity. Membranes are better suited for detection of colorimetric or chemiluminescence reporters. Pall introduced a similar product, the Vivid GeneArray slide, based upon nylon-impregnated slides. 

Advantages — Optical transparency and flatness low intrinsic fluorescent backgrounds light scattering ultraflatness impervious surface allows high density array construchon. 

Muller MG, Georgakoudi I, Zhang QG, Wu J, Feld MS. Intrinsic fluorescence spectroscopy in turbid media disentangling effects of scattering and absorption. Applied Optics 2001, 40, 4633 1646. 

In these sensors, the intrinsic absorption of the analyte is measured directly. No indicator chemistry is involved. Thus, it is more a kind of remote spectroscopy, except that the instrument comes to the sample (rather than the sample to the instrument or cuvette). Numerous geometries have been designed for plain fiber chemical sensors, all kinds of spectroscopies (from IR to mid-IR and visible to the UV from Raman to light scatter, and from fluorescence and phosphorescence intensity to the respective decay times) have been exploited, and more sophisticated methods including evanescent wave spectroscopy and surface plasmon resonance have been applied. 

Another type of intrinsic property is derived from the theory of light scattering in particles. The phenomenon of Raman and fluorescent scattering from molecules suspended in small dielectric particles exemplifies such prop-... 

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