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Scale-up of HNL-Catalyzed Cyanohydrin Formation

Important issues Laboratory Scale up Industrial production [Pg.160]

Source of enzyme Natural source Recombinant protein developed High expression and cheap enzyme [Pg.160]

Achieve high ee Use bi-phasic system, emulsion, check pH, X concentration Use bi-phasic system and intense stirring to form an emulsion Use bi-phasic system and intense stirring without shearing of enzyme (P 3kW/m3) [Pg.160]

Optimal conditions Use diisopropyl ether Use MTBE, H3P04 No temperature con- [Pg.160]

Phase separation Use filter aid Filtration, centrifugation Optimize stirring regime [Pg.160]

See other pages where Scale-up of HNL-Catalyzed Cyanohydrin Formation is mentioned: [Pg.159]   


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Cyanohydrin formation

Cyanohydrine

Cyanohydrins

Scale formation

Scale-up

Scale-ups

Up scaling

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