Scaffolds osteoblasts-cultured

A. S. Goldstein, G. Zhu, G. E. Morris, R. K. Meszlenyi, and A. G. Mikos, Effect of osteoblastic culture conditions on the structure of poly(DL-lactic-co-glycolic acid) foam scaffolds, Tissue Eng. 5, 421-433 (1999). 

DBD Plasma Effect on Attachment and Proliferation of Osteoblasts Cultured over Poly-e-Caprolactone Scaffolds... 

Ishaug, S. L. et al.. Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds, J. Biomed. Mater. Res., 36,17,1997. 

Figure 3. These images represent the types of structural and chemical information that can be obtained using the OCM-CFM dual-mode technique (a,), CARS (b.f and immersive visualization (cf The OCM-CFM images (a) are of fetal chick osteoblasts cultured on porous polycaprolactone scaffolds. Differences in pore size and crystalline regions of the polymer could be determinied at 145 pm from the surface. Figure b is a broadband CARS micrograph of a phase-separated ternary polymer blend. The colors green, blue, and red represent polystyrene, poly(ethylene terphthalate) and poly(methyl methacrylate), respectively. Figure c is a 3D respresentation of cells on polycaprolactone scaffold taken from the immersive visualization laboratory. This visual image provides additional information on cell shape, orientation, and position within the scaffold.

MTT tests (11) and SEM analysis of the osteoblasts-cultured scaffolds could confirm the biocompatibility of the composite scaffolds. 

Alvarez-Barreto, J. E, L. Bonnie, S. VanGordon et al. 2011. Enhanced osteoblastic differentiation of mesenchymal stem cells seeded in RGD functionalized PLEA scaffolds and cultured in a flow perfusion bioreactor. ] Tissue Eng Regen Med 5(6) 464-75. 

In the earhest stages of bone engineering, osteoblasts were used to prove the concept of bone regeneration in two ways osteoblasts cultured in vitro on scaffold materials produced bone-like matrix, and... 

Gough, JJ ., Jones, J.R., and Hench, LX. (2004) Nodule formation and mineralisation of human primary osteoblasts cultured on a porous bioactive glass scaffold. Biomaterials, 25, 2039-2046. 

Human osteoblast-like MG63 cells were cultured on the macroporous chitosan scaffolds reinforced with hydroxyapatite or calcium phosphate invert glass were fabricated using a thermally induced phase separation technique. 

Figure 27. Human osteoblast-like MG 63 cells in cultures on porous (A) or fibrous (B) poly(L-lactide-co-glycolide) scaffolds. A A summarizing picture of horizontal optical sections. The depth of cell ingrowth into the pores (average pore diameter of 400-600 mm) is indicated by spectral colors (blue 0-60 mm, green 80-160 mm, yellow 180-220 mm, orange 240-300 mm, red 320-400 mm, violet 420-480 mm). Day 14 after seeding, cells stained with propidium iodide. B cells grown for 4 days in static culture followed by 2 days in dynamic perfusion cell culture system. Cell membrane stained with Texas Red C2-maleimide and the nuclei counterstained with Hoechst 33342. Leica TCS SP2 confocal microscope, objective 5x (A) or lOx (B) [37].

Here, we describe methods to evaluate the ability of small molecules inhibitors of TNAP and PHOSPHOl in preventing mineralization of primary cultures of murine vascular smooth muscle cells. The procedures are also applicable to primary cultures of calvarial osteoblasts. These cell-based assays are used to complement kinetic testing during structure-activity relationship studies armed at improving scaffolds in the generation of pharmaceuticals for the treatment for medial vascular calcification. 

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