Gene-delivery scaffolds

In the following sections a detailed analysis of the different hyperbranched scaffolds commonly used in gene delivery is described. Several examples are... 

Several amine functionalized hyperbranched PGs have been reported as potential gene delivery systems after a proper surface group functionalization. In comparison to other dendritic structures, these scaffolds have the added advantage of being open, flexible, and possessing a polyether backbone which keeps the toxicity profile low. Different systems have been studied by post-modification of the hydroxyl groups... 

The search for an efficient and non-toxic gene transfection vector has led to the design and synthesis of a great variety of macromolecular scaffolds. An extensive analysis of the key features for the efficient and safe delivery of genes in vivo and in vitro has led to the conclusion that hyperbranched polymers are potential candidates for further development. In this chapter we have presented a detailed analysis of the different hyperbranched polymer scaffolds commonly used in gene delivery applications. Several structural modifications toward the development of an optimal gene vector have been analyzed. 

Carbohydrate structures can also act as a scaffold for dendrimer formation. The polysubstituted sugar 23 (Fig. 5) has been synthesized and shown that it can act as a scaffold onto which peptide sequences can be assembled to form another novel vaccine system (15). The development of this carbohydrate scaffold technology and its application to vaccine and gene delivery is under way. The synthesis of 23 is complex a complete account of the procedures can be found in ref. 15. 

Keywords Antibiotics Biomolecules Cancer Drug delivery Electrospinning Gene delivery Nanofibers Scaffolds Tissue engineering... 

Jang J H, et al. (2006). Surface adsorption of DNA to tissue engineering scaffolds for efficient gene delivery. J. Biomed. Mater. Res. A. 77 50-58. 

Some of the most interesting properties of PGA that make it an ideal material for biological applications are its molecular weight, viscosity, biodegradability, and most importantly its vast availability[237,240,244]. It has found profound applications in the areas of development of scaffolds, drug and gene delivery, and tissue adhesive and sealant and coatings for several applications. Some of the... 

J.A. Smolen, Emulsion electrospinning fa- producing dome-shaped structures within 1-tyrosine polyurethane scaffolds for gene delivery. Master s Thesis, The Univosity of Akron, Akron, OH, 2010. 

L. De Laporte, L.D. Shea, Matrices and scaffolds for DNA deUvery in tissue engineering, Adv. Drug Deliver. Rev. 59 (2007) 292-307. J.H. Jang, XL. Houchin, L.D. Shea, Gene delivery from polymer scaffolds for tissue engineering. Expert Rev. Med. Devices 1 (2004) 127-138. 

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