Saturation transfer spectroscopy

Hyde, J.S. and Dalton, L.R. 1979. Saturation-transfer spectroscopy. In Spin labeling II Theory and Applications, ed. LJ. Berliner. New York Academic Press. 

E. L. Lim, K. G. Hollingsworth, P. E. Thelwall and R. Taylor, Measuring the acute effect of insulin infusion on ATP turnover rate in human skeletal muscle using phosphorus-31 magnetic resonance saturation transfer spectroscopy. NMR Biomed., 2010, 23,952-957. 

C. Nabuurs, B. Huijbregts, B. Wieringa, C. W. Hibers and A. Heerschap, P saturation transfer spectroscopy predicts differential intracellular macromolecular association of ATP and ADP in skeletal muscle. /. Biol. Chem., 2010, 285,39588-39596. 

Marsh D, Horvath L, Pah T, Livshits VA. Saturation transfer spectroscopy of biological membranes. In Biomedical ESR. Eaton GRESS, Berliner LJ, eds. 2004. Kluwer Academic/Plenum Publishing, New York. pp. 309-368. 

A very important ELDOR mechanism is diffusion of saturation because of very slow rotational diffusion of nearly immobilized species. This is the basis of saturation transfer spectroscopy [57]. 

Correlation times with time scales of 10 to 10 s are determined by the above method and are said to be in the fast tumbling region. In the slow tumbling region (lO" to 10 s) more sophisticated theoretical approaches [79], [80] are used. The very slow region (1 O to 10 s) cannot be studied by conventional ESR and requires special techniques such as saturation transfer spectroscopy (see Section 18.6.5.4). 

Saturation Transfer Spectroscopy of Spin-Labels Techniques and Interpretation of Spectra... 

Mayer, M., Meyer, B. Characterization of ligand binding by saturation transfer difference NMR spectroscopy. Angew. Chem. Int. Ed. 1999, 38, 1784-1788. 

X-ray crystallography, docking modes can be validated by various NMR techniques NOEs may be observed between the ligand and the receptor protein by heteronuclear-filtered NOE spectroscopy [51], chemical shift changes of protein resonances upon binding can be analyzed by simulation of shifts caused by ring currents and electrostatic effects [52], and saturation transfer difference measurements indicate which part of the ligand is in direct contact with the protein [52]. 

Data obtained by ITC experiments were corroborated by saturation transfer difference (STD) NMR spectroscopy. Signals that arise during an STD experiment are a... 

M. Schar, A.-M. M. El-Sharkawy, R. G. Weiss and P. A. Bottomley, Triple repetition time saturation transfer (TRiST) P spectroscopy for measuring human creatine kinase reaction kinetics. Magn. Reson. Med., 2010, 63,1493-1501. 

Di Micco, S., el al., Differential-frequency saturation transfer difference NMR spectroscopy allows the detection of different ligand-DNA binding modes. Angew Chem Int Ed, 2006, 45, 224-228. 

NOESY/STD Nuclear Overhauser Enhancement spectroscopy/saturation transfer difference... 

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