Sanger dideoxy method

Figure 28.8 The sequence of a restriction fragment determined by the Sanger dideoxy method can be read simply by noting the colors of the dye attached to each of the various terminal nucleotides.

Sequencing of DNA is carried out by the Sanger dideoxy method, and small DNA segments can be synthesized in the laboratory by automated instruments. Small amounts of DNA can be amplified by factors of 106 using the polymerase chain reaction (PCR). 

Sanger dideoxy method (Section 2.6) The most commonly used method of DNA sequencing. 

Correct answer = B. The sequence of the new strand of DNA synthesized using the Sanger dideoxy method may be determined by reading the bands from the bottom to the top of the gel as 5TACCAG3 This is complementary to the original strand, which is. therefore, 5 CTGGTA3 ... 

Common method of DNA sequencing Cloned, purified fragments of DNA can be sequenced using the Sanger dideoxy method. 

What are the requirements for sequencing DNA with the Sanger dideoxy method ... 

DNA sequencing Maxam-Gilbert method restriction endonuclease restriction fragment palindrome Sanger dideoxy method DNA synthesis DMT ether phosphoramidite phosphite polymerase chain reaction (PCR)... 

Sanger dideoxy method (Section 28.6) an enzymatic method for DNA sequencing. 

Figure 5-21. DNA sequencing by the Sanger-dideoxy method. Single or double stranded DNA is used as a template for a primer-dependent DNA polymerase extension, which is carried out in four separate reaction mixes. In addition to DNA polymerase and the four dNTPs (including an a-radiolabelled nucleoside triphosphate), each reaction mix contains a small proportion of one dideoxy nucleotide derivative, ddATP,...

DNA Is Usually Sequenced by Controlled Termination of Replication (Sanger Dideoxy Method)... 

Rapid sequencing techniques have been developed to further the analysis of DNA molecules. DNA can be sequenced by controlled interruption of replication (Sanger dideoxy method). The fragments produced are separated by gel... 

All large-scale DNA sequencing is now done by the Sanger dideoxy method, which has several different variants. One particularly important variant used in commercial sequencing instruments begins with a mixture of the following ... 

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