Sample preparation homogeneous

Sample preparation Homogeneity testing Stability testing... 

Sample preparation Homogenize sample, wei out sample equivalent to 50 mg cefadroxil, make up to 50 mL with 100 mM pH 4.5 phosphate buffer. Take 5 mL of this solution, add 0.5 mL 30 mg/mL dimethyl phthalate in MeCN water 1 1, make up to 50 mL with 100 mM pH 4.5 phosphate buffer. 

Sample preparation Homogenize muscle with three volumes phosphate-buffered saline (Pol3d ron, level 3) for 2 min, centrifuge at 1300 g for 10 min. 125 p,L Supernatant + 100 (jiL water + 800 jjlL MeCN, vortex for 30 s, centrifuge at 1600 g for 5 min. Remove the supernatant and evaporate it to dryness under a stream of nitrogen, reconstitute the residue in 125 irL mobile phase, inject a 50 xL aliquot. 

Sample preparation Homogenize (Polytron PCU-2) 150-200 mg skin with 4 mL chloroform, repeat homogenization, filter (phase-separating paper) extracts. Make the residue alkaline with 2 mL 10% NaOH, extract twice with 4 mL portions of chloroform, wash the extracts twice with 2 mL portions of water, filter (phase-separating paper) the organic layer. Combine all the chloroform layers and evaporate them to dr3mess under a stream of air, reconstitute the residue in 1 mL mobile phase, filter (microfilter), inject an aliquot. 

Sample preparation Homogenize liver at 20 mg/mL in 50 mM pH 7.4 TWs-HCl buffer. 200 (xL Plasma or 250 p.L liver homogenate + 250 ng IS -i- pH 7.3 ammonium phosphate buffer -I- MTBE, extract. Remove the organic layer and add it to 250 xL 50 mM phosphoric acid, extract. Remove the aqueous layer and add it to 100 pL MeCN, inject an aliquot. 

Sample preparation Homogenize 1 mL liver in 5 mL water. Centrifuge 1 mL homogenate,... 

Sample preparation Homogenize in 4 mL water, add 4 mL ice-cold acetone, centrifuge at 3000 rpm for 15 min, extract twice with an equal volume of hexanes. Evaporate extracts under vacuum, reconstitute in n-propanol, inject an aliquot. 

Sample preparation Homogenize tissue 1 2 (w/v). 1 mL Sample + 100 jjiL 10 jig/mL methaqualone + 1 mL ammonium chloride/eimmonium hydroxide buffer (pH 9.2) -I- 3 mL n-butyl chloride, mix, centrifuge. Remove the organic layer and evaporate it to dryness under nitrogen at 45°, reconstitute the residue with 50 p,L mobile phase, inject a 20 p,L aliquot. 

Sample preparation Homogenize (Tekmar Tissuemizer SDT 1810 with SDT 182 EN shaft/blades) 5 g finely chopped muscle tissue and 20 mL MeCN at medium speed for 45 s, centrifuge at 1500 g for 5 min, add 15 mL MeCN-saturated hexane, shake for 30 s, discard the hexane layer, repeat the wash. Add 9 mL EtOH to the MeCN layer and evaporate under reduced pressure to 2-5 mL at 45°, add 2 mL EtOH, evaporate to 2 mL, add 2 mL EtOH, evaporate to dryness, add 1 mL mobile phase, sonicate for 5 min, centrifuge at 15400 g for 10 min, filter (0.45 pm) the supernatant, iiyect a 50 pL aliquot of the supernatant. 

Sample preparation Homogenize (Omni-mix) 50 mg bark or foliage in 1.5 mL MeOH for 2 min, sonicate for 5 min, centrifuge, filter (0.2 pm) the supernatant, iiyect an aliquot of the filtrate. 

Sample preparation Homogenize (Polytron Model PT-1200C) 1 mL whole blood or whole rat brain with 1 mL water for 3-5 min, add 4 mL MeCN, vortex, add 1 mL concentrated saline, allow to settle at -5° for 1 h. Remove the organic layer, filter, dilute 1 4 with 50 mM ammonium acetate, inject an aliquot. 

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