Sambucus lectins

Recently, a lectin made from purified elderberry (Sambucus nigra) has been used in the fractionation of normal bone marrow cells. Fractionation minimizes the effects of radiation on normal tissue. This work has been carried out by Dr. Madeleine Mumcuoglu, who patented the elderberry flu formulas, as well as doctors Daphna Manor and Shimon Slavin. There is hope that elderberry contains properties that may have potential in improving the success of bone marrow transplants. 

Several carbohydrate-binding proteins have been isolated from Sambucus nigra and two haemagglutinins have been isolated from its bark. Its seeds contain a lectin that is related to the immunological properties of the bark. One of its carbohydrate-binding proteins, called nigrin, is made of two subunits and is reported to cause red blood cells to clump. 5... 

Fig. (25). Periodate oxidation (A) and reaction with lectins (B) of anti-erythropoietin antibodies (S), EO, E2, E8= hrs of oxidation, LI = Maackia, L2 = Sambucus.

Lectin PNA Arachis hypogaea Lectin RCA 120 Ricinus communis Lectin SNA Sambucus nigra... 

Carbohydrate chains of Cl-inhibitor were identified by a binding assay using different lectins [36]. Lectins from Sambucus nigra (SNA) and Maackia amurensis (MAA), that are specific for sialic acids, were shown to bind to Cl-inhibitor. Lectin from Datura stramonium (DSA) also reacted with the inhibitor indicating complex and hybrid sugar structures. Cl-inhibitor was enzymatically desialylated and reexamined for lectin binding. SNA and MAA did not react anymore, but in addition to DSA, peanut agglutinin, which can bind to carbohydrate chains after sialic acids are removed, bound to desialylated Cl-inhibitor. Cl-inhibitor contains about 30 sialic acid residues per molecule. SDS-... 

Fig. 13 Glycoprofiling of placental and glycoengineered hCG based on the binding of specific lectins. Lectins specific for a2,3/6-bound sialic acids (black bars SNA, Sambucus nigra agglutinin), a2,6-bound sialic acids (grey bars PSL, Polyporus squamosus lectin) and mannose-rich oligosaccharides (white bars GNL Galanthus nivalis lectin).

Asia and Mongolia, Boehringer 1206 826) and a lectin (SNA) from the bark of Elder (Sambucus nigra, Boehringer 1206 940) . After densitometry of the Coomassie Blue stained bands (CBB, Fig. 1) and of the lectin stained bands (Fig. 2), densitometer values in arbitrary units were determined. A glycoprotein to protein ratio (G = LEG CBB) was calculated. This ratio G was taken as a measure for the intensity of glycation, which is characteristic for each purified protein, as will be shown for example by horseradish peroxidase (HRP). 

Van Damme, E.J., Barre, A., Rouge, R, Van Leuven, F. and Peumans, W.J., The NeuAc(alpha-2,6)-GaFGalNAc-binding lectin from elderberry Sambucus nigra) bark, a type-2 ribosomeinactivating protein with an unusual specificity and structure. Eur. J. Biochem., 235, 128-137 (1996). 

Girbes, T., Chores, L., de Benito, F.M., Inglesias, R. and Ferreras, J.M., A non-toxic two-chain ribosome-inactivating protein co-exists with a structure-related monomeric lectin (SNA IB) in elder Sambucus nigra) fruits. Biochem. J., 315, 343-344 (1996). 

Compounds from Sambucus species contain a number of plant lectins with hemagglutinin characteristics, which could prove useful in blood typing and hematological tests. ... 

---