Stain lectin

The hydrophobic PVDF membranes are mechanically and chemically stable. They are suited for protein staining, immunostaining, lectin staining, and Ca + staining, as well as for amino acid sequencing and analyses. Similar conditions apply to blot and development as to nitrocellulose, but in contrast to nitrocellulose the PVDF membrane does not completely bind even tiny amounts of protein. Depending on the protein, 10 to 50% passes the membrane unbound. Watery solutions do not moisten dry PVDF membranes. The membranes first have to soak in methanol. Moist PVDF membranes are light-permeable and transparent in dioxan isobutanol. Stained bands can also be measured in the scanner. 

REGIONALITY OF GLYCATED CALF LENS CRYSTALLIN SUBUNITS DEMONSTRATED BY LECTIN STAINING... 

Asia and Mongolia, Boehringer 1206 826) and a lectin (SNA) from the bark of Elder (Sambucus nigra, Boehringer 1206 940) . After densitometry of the Coomassie Blue stained bands (CBB, Fig. 1) and of the lectin stained bands (Fig. 2), densitometer values in arbitrary units were determined. A glycoprotein to protein ratio (G = LEG CBB) was calculated. This ratio G was taken as a measure for the intensity of glycation, which is characteristic for each purified protein, as will be shown for example by horseradish peroxidase (HRP). 

The obtained results are specific for glycoproteins, as glycolipids were washed out during the histological treatment and thus have not to be considered here any further. The overall results on lectin affinity to the human conjunctival goblet cells are summarize in Table 2. As documented there, lectin staining was observed with all but LPA and SNA. 

Paul, A., Ulfig, N. Lectin staining in the basal nucleus (Meynert) and the hypothalamic tuber-omamillary nucleus of the developing human prosencephalon. Anat. Rec., 1998, 252, 149-158. 

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