Salmon Sperm and Calf Thymus DNA

6 Raman Studies on the Reaction of MYKO 63 with Salmon Sperm and Calf Thymus DNA 

It should noted that the N(3) position of adenine is not a binding site for MYKO the 1580-cm band, which is very sensitive to binding to the adenine base in the N(3) position shows no significant shift or decrease either in frequency or in intensity in any of the spectra. 

The fact that adenine appears to be the target for MYKO 63 through a dialkylating process on the N(7) and NHj sites is consistent with the very low kinetics of complexation of MYKO 63 to DNA mentionned above Lawley and Brookes Maxam and Gilbert and Goodwin et al. have clearly demonstrated that methylation on adenine happens very slowly, five times slower, for example, than on guanine. 

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