Saccharomyces cerevisiae principle

It was tempting to base the study of membrane transport in eukaryotic cells on similar simple principles. For this purpose, as well as for molecular biology as a whole, the yeast Saccharomyces cerevisiae appeared to be the best suited organism. From early times on, this yeast has occupied a privileged place for mankind. Due to... 

Raamsdonk and colleagues set out to develop a metabolomics method that could be used to characterize proteins of unknown function in yeast [14]. Using an NMR approach they analyzed intracellular metabolites in mutants of Saccharomyces cerevisiae. The resulting NMR spectra were then analyzed by multivariate analysis, including principle component analysis (PCA) to identify differences in the spectra that can distinguish different mutants (Fig. 2). Two important results came out of these studies that would reveal the value of metabolomics in biological research. 

Ihmels J, Levy R, Barkai N. Principles of transcriptional control in the metabolic network of Saccharomyces cerevisiae. Nature Biotechnol. 2003 22 86-92. 

PathDB (table 7.1) has been under development since before 2000 at the National Center for Genome Resources. It focused originally on plant metabolism (Arabi-dopsis thaliana) but is not in principle restricted to plants, and has recently begun including yeast (Saccharomyces cerevisiae). It includes a relatively large database of compounds, proteins, reactions, and pathways, plus visualization and search/nav-igation tools. 

Kuyper, M., Winkler, A. A., Van Dijken, J. P., Pronk, J. T. (2004). Minimal metabolic engineering of Saccharomyces cerevisiae for efficient anaerobic xylose fermentation a proof of principle. FEMS Yeast Research, 4, 655-664. 

FIGURE 5.5 Principle of measurement using recombinant Saccharomyces cerevisiae [38]. 

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