Running a preparative hplc column

The sequence of events for running a preparative hplc column is almost the same as that for mplc, as described above. However, there are a few significant differences which should be taken into account, and will be described here. 

First of all you should appreciate that preparative hplc should only be used for separation of clean mixtures. Some other form of chromatography should always have been carried out previously to remove any material which is significantly more polar than the compounds to be separated and any suspended solid should also be removed. Preparative hplc is a very powerful technique and it is often possible to separate compounds which do not separate on tic. However, preparative hplc columns are extremely expensive, and although they should last for a very long time, they can 

For full-scale preparative work either a 22.5mm or a 45mm column is ormally used and it is easy to scale up to this size knowing the conditions used for the smaller column. If the columns are the same length the scale-jp factor wiU depend on the area of the column surface, thus - 

Scale-up factor, F = Area(large col) = 7tR2(large) = (D/2)2(large) Area(small col) 7tR2(small) (D/2)2(small) 

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