Rumen inoculum

Figure 9. Wood pulps of various yields. Relationship between median pore size and the amount of cellulose dissolved in 24 hours by a rumen inoculum...

J. Stone Well, we dry the material in the oven overnight, and then soak it in water for 24 hours before the digestion with the rumen inoculum. Our dried material is material which has been dried and re-swollen in water. ... 

Figure 2. Effect of activity of rumen inoculum on digestion ceilings of aspen silage...

Miller SM, KUeve AV, Plumb JJ, Blackall LL (1997) An in vitro cultured rumen inoculum improves... 

Table II. Growth of Rumen Bacteria on inoculum) Sugar Beet Pulp, (5%...

The aspen silage was extremely low in nitrogen (0.1% DM basis) so that nitrogen deficiency in the inoculum from animals fed this material was probably a major factor in reducing the cellulolytic activity of the rumen microflora. 

Tableii shows a comparison between fermentability values utilizing rumen and human inocula on the same substrates. A comparison of this nature may have inherent inequalities due to differences in inoculum source and collection procedures. These parameters have been evaluated by a number of workers (11, 17, 18). The differences in nutritional schemes between animal species have an effect on microbial populations. Fermentability estimates of a particular substrate are biologically acceptable with respect to the inoculum source.

Fermentability using human fecal inoculum ranges from a high of 91% (cabbage) to a low of 0% (Maillard product) demonstrating the sensitivity of human microflora to different fiber sources. Fermentability of cellulose by human fecal microflora (23Z) is substantially less than rumen microflora (94%). The microflora in the human intestine may be more affected by fiber composition than rumen microflora. Work by Bryant (19) and Jeraci (11) leads to speculation that variation among inoculum sources in humans on a particular substrate could be greater than in other species. 

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