Rrans-Resveratrol

For the red wines (82-84), which were injected directly into the HPLC without sample preparation, a ternary-gradient system using aqueous acetic acid (1% and 5% or 6%), and acidified acetonitrile (acetonitrile-acetic acid-water, 30 5 6) was used for cinnamic acid derivatives, catechins, flavonols, flavonol glycosides, and proanthocyanidins. Due to the large number of peaks, the gradient was extended to 150 min for the resolution of many peaks of important phenolics. This direct injection method was able to separate phenolic acids and esters, catechins, proanthocyanidins, flavonols, flavonol glycosides, and other compounds (such as tyrosol, and rrans-resveratrol) in wine in a single analysis. However, use of acetic acid did not permit the detector (PDA) to be used to record the UV spectra of phenolics below 240 nm (84). 

As an example, we shall study here the application of a laser technique specially designed to perform fast and direct analysis of non-volatile compounds in fmit and vegetables, particularly rrans-resveratrol (3,5,4 -trihydroxystilbene) in grapes and vine leaves. The method is based on the combination of LD followed by REMPI and TOFMS detection, often identified by its sum of acronyms, i.e. LD-REMPI-TOFMS (e.g. Orea et al, 2001, and Orea and Gonzalez Urena, 2002)... 

Fig. 51.3 Stilbenes accumulation in 35SS and LoxS whole mature red fruits (a) and skin (b). The amount of /rans-resveratrol, rrans-piceid, and their correspondent cis-foims were simultaneously detected on the basis of their retention time and UV spectra of the additional peaks observed after UV irradiation at X 366 nm of rrans-resveratrol and rraws-piceid, respectively...

Trans-R, 1706, 1707 Jrani-resveratrol, 1569, 1685 rrans-stilbene, 1553 Trans-zeatin, 958 rrans-zeatin-type cytokiniiis, 956 TR-DNA, 2948 Trechonaetes, 3467 Treemoss, 3299 Trehalose, 2646 Tri5, 3130, 3131 TrilOl, 3131... 

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