Rotation planar chromatography stationary phase

The main difference between the first four methods [normal chamber RPC (N-RPC), microchamber RPC (M-RPC), ultra-microchamber RPC (U-RPC), and column RPC (C-RPC) 1 lies in the size of the vapor space, that is an essential criterion in rotation planar chromatography. The circular mode of development is always used for on-line preparative separations by all of these methods. The sample is applied near the center of the layer, and the mobile phase is forced through the stationary phase from the center to the outside of the round plate/planar column. The separated compounds are eluted from the layer/planar column as a result of the centrifugal force and collected in a fraction collector. 

Complementary to paper chromatography, but used more frequently because of the wide availabihty of stationary phases, is thin layer chromatography (TLC). It is simple, fast, reproducible and can achieve high resolution. It is usually performed on a square plate or on strips. A variation of this type of planar chromatography is carried out on a rotating circular plate using an instrument called a Chromatotron. 

Kalasz et al. used spacer to improve separation of radiolabeled metabolites, and the method was called spacer D-TLC. They also constructed specific parameters of DC, especially for calculation of the resolution (Rq), yield (7), loss (L), and efficiency ( ). Displacement of radiolabeled compounds was readily visualized using X-ray film with contact autoradiography. Two-dimensional chromatography can be simply arranged when a planar stationary phase is used. The stationary phase must be rotated 90° after the 1st dimensional development then, the system is ready for the 2nd dimensional run. Elution type of development followed by displacement is an easy and useful means of two-dimensional thin-layer chromatography... 

---