Rocking shaker

If not noted elsewhere, all washing, incubation, and reaction steps could be performed using a rocking shaker. 

Rtlttelbew ung (schneU bin und her/rauf-runter) rocking shaker (see-saw motion)... 

A shaker tube equipped with a 1200-atm. rupture-disk assembly was used by the submitters. The checkers used a 1270-ml. stainless steel rocking autoclave fitted with a thermocouple well that extended into the reaction mixture and a stainless steel 5000-p.s.i. rupture disk. The agitation rate was 58 cycles per second. Attempts to use a magnetically stirred autoclave were unsuccessful. 

Place membrane in heat-sealable plastic bag with 5 ml blocking buffer and seal bag. Incubate 30 min to 1 hr at room temperature with agitation on an orbital shaker or rocking platform. 

Equilibrate membrane in appropriate blocking buffer in heat-sealed plastic bag with constant agitation using an orbital shaker or rocking platform. For nitrocellulose and PVDF, incubate 30 to 60 min at room temperature. For nylon, incubate >2 hr at 37°C. 

A reaction vessel equipped with a fritted disc, a solvent inlet, and a device to transfer in and out liquids under low nitrogen pressure was used to carry out solid-phase syntheses. This manual apparatus enables suspensions of resin to be stirred either by gently rocking the vessel on a shaker or by bubbling nitrogen through the fritted plate. 

Donovan, T.J., Roberts, A.A. and Dalziel, M.C., 1981. Epigenetic zoning in surface and near-surface rocks resulting from seepage-induced redox gradients, Velma oil field, Oklahoma a synopsis. Oklahoma City Geological Society Shale Shaker, 32(3) 1-7. 

In another experiment several grams of crushed unweathered rock from the watershed were placed in distilled water in a polyethylene bottle. In a second bottle, several grams of fresh rock were placed in stream water. The bottles were then placed on a shaker table, and the waters were analyzed periodically. Within a week the distilled water had achieved a composition similar to the stream (less rain water contribution) and the stream water had not changed (Table V). We conclude from the experiments that the water reacts rapidly with the silicate minerals and the final concentrations of the dissolved constituents in the water are controlled by the silicate minerals. (The results of these experiments and data for a group of individual minerals will be presented in detail in a separate publication.)... 

In addition to the above, the potential of a bacterial problem in the reservoir appears acute because of the species which were isolated in API shaker tubes and which were microphotographed in the SEM work on random samples of reservoir rock. This SEM microphotograph is illustrated in Figure 27. The rods shown in the microphotograph are very similar to the photomicrographs of bacteria [0.3 to 0.5 micron diameter by 0.7 to 2.0 micron length (10)] associated with Kelzan M.F. biopolymer. Such bacteria can cause wellbore impairment and reduced injectivity (10). 

Dissolve 100 mg of DTT in 10 ml of equilibration buffer (= equilibration buffer I). Take out the focused IPG gel strips from the freezer and place them into individual test tubes (200 mm long, 20 mm i.d.). Add 10 ml of equilibration buffer I and 50 p of the bromphenol blue solution. Seal the test tubes with Parafilm, rock them for 15 min on a shaker (Fig. IF), and then pour off the equilibration buffer. 

Rocking platform shaker (e.g., BC-720 BIO CRAFT, EverSeiko Corporation, Tokyo, Japan). 

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