Robustness fractionation/purification

Example 2 Robustness of Pasteurization and Precipitation Steps in a Fractionation/Purification Process... 

The production process for protein prepared by fractionation/ purification includes a heat inactivation step to ensure viral safety of the product. The heat inactivation step is followed by a precipitation step that further purifies the product. The purpose of our study was to determine whether the process steps are robust within the parameter ranges routinely used in manufacturing. 

Commercialization of semiochemicals for use in crop protection or in the fight against invasive weeds has an important cost issue. A conq)any must make a profit on any business activity in order to survive in a highly competitive marketplace. For this reason, synthetic pathways to the target compounds must be short and contain robust reactions. Elegant but difficult chemistry must be replaced by operationally convenient reactions. Tedious and expensive fractional distillation or chromatographic purification must be eliminated or at least minimized. These Tacts of life are usually neglected in the synthetic chemical literature so they are presented here. 

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