Agglutinins Ricinus communis

There is still some discordance about markers for ATI cells [17, 18]. Varieties of glycocalyx appear to be expressed in ATI versus ATII cells, which can be distinguished by the binding of specific lectins. Lycopersicon esculen-tum lectin, for example, appears to adhere specifically to the apical plasma membrane of ATI cells [19, 20], Other lectin markers that have been reported in AEC typing are Ricinus communis agglutinin, Erythrina cristagalli lectin, soybean lectin and Bauhinia purpurea agglutinin (all for the ATI phenotype),... 

Nicolson, G.L., Blaustein, J. (1972). The interaction of Ricinus communis agglutinin with normal and tumor cell surfaces. Biochim. Biophys. Acta. 266 543-7. 

Mannoji H, Yeger H, Becker LE (1986) A specific histochemical marker (lectin Ricinus communis agglutinin-1) for normal human microglia, and application to routine histopathology. Acta Neuro-pathol (Berl) 71 341-343. 

Chen, G.Q., He, X. and McKeon, T.A. (2005) A simple and sensitive assay for distinguishing the expression of ricin and Ricinus communis agglutinin genes in developing castor seed R. communis L.). J Agric Food Chem, 53, 2358-2361. 

Hegde, R. and Fodder, S.K. (1998) Evolution of tetrameric lectin Ricinus communis agglutinin from two variant groups of ricin toxin dimers. Eur J Biochem, 254, 596-601. 

Pinkerton, S.D., Rolfe, R., Auld, D.L., Ghetie, V. and Lauterbach, B.F. (1999) Selection of castor for divergent concentrations of ricin and Ricinus communis agglutinin. Crop Sci, 39, 353-357. 

Sphyris, N., Lord, J.M., Wales, R. and Roberts, L.M. (1995) Mutational analysis of the Ricinus lectin B-chains. Galactose-binding ability of the 2 gamma subdomain of Ricinus communis agglutinin B-chain. J Biol Chem, 270, 20292-20297. 

Lord JM (1985a). Precursors of ricin and Ricinus communis agglutinin. Glycosylation and processing during synthesis and intracellular transport. Eur J Biochem, 146, 411 116. 

Roberts LM and Lord JM (1981). The synthesis of Ricinus communis agglutinin, cotranslational and posttranslational modification of agglutinin polypeptides. Eur J Biochem, 119, 31—41. 

Ricinus communis agglutinin (RCA-120) 4% cross-linked Agarose (Sigma) 0.3 M P-methyl-galactopyranoside ... 

C. L. Schofield, B. Mukhopadhyay, S. M. Hardy, M. B. MeDonnell, R. A. Field, and D. A. Russell, Colorimetric detection of Ricinus communis agglutinin 120 using optimally presented carbohydrate-stabilised gold nanopartieles. Analyst, 133 (2008) 626-634. 

The binding of Ricinus communis agglutinin to the mitochondrial inner membrane now appears to be an artefact of the preparation of the R. communis mitochondria. When endosperms from R. communis are homogenized in the presence of H-labelled R. communis agglutinin, with and without the addition of lactose, the labelled lectin is only found associated with the mitochondrial membrane fraction in the absence of lactose. 

Allyl a-L-rhamnoside, immobilized by copolymerization with acrylamide and AW -methylene bisacrylamide, has provided a matrix for the large-scale affinity chromatographic separation of Ricinus communis agglutinin (which binds) and toxin (which does not bind). A simple method has been developed for the... 

Affinity chromatography on immobilized Ricinus communis agglutinin has been used for the isolation of a heterogeneous population of human serum inhibitors of the binding of desialylated glycoproteins to rat hepatocyte membranes. The inhibitors are themselves desialylized glycoproteins, which are present in small amounts in normal human serum but in increased levels in serum from patients with cirrhosis. 

Spain SG, Cameron NR (2011) The binding of polyvalent galactosides to the lectin Ricinus communis agglutinin 120 (RCA(120)) an ITC and SPR study. Polym Chem 2(7) 1552-1560... 

Mizukami K, Takakura H, Matsunaga T, Kitano H (2008) Binding of Ricinus communis agglutinin to a galactose-carrying polymer brush on a colloidal gold monolayer. Colloid Surf B 66(1) 110-118... 

Butterworth, A.G., Lord, J.M., 1983. Ricin and Ricinus communis agglutinin subunits are all derived from a single-size polypeptide precursor. Eur. J. Biochem. 137, 57-65. 

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