RIANA immunosensors

Among this variety of immunosensor systems, two optical immunosensor devices for environmental applications RIANA and AWACSS are especially noteworthy. These immunosensors are based on solidphase fluoro-immunoassays combined with an optical transducer chip chemically modified with an analyte derivate. The RIANA immunosensor was applied successfully in a variety of environmental applications (Mallat et al., 1999 Rodriguez-Mozaz et al., 2004b). The other new analytical system AWACSS has... 

S. Rodriguez-Mozaz, S. Reder, M. Lopez de Alda, G. Gauglitz, and D. Barcelo, Simultaneous multianalyte determination of estrone, isoproturon and atrazine in natural waters by the River ANAlyser (RIANA), an optical immunosensor. Biosens. Bioelectron. 19, 633-640 (2004). 

Rodriguez-Mozaz S, Lopez de Alda MJ, Barcelo D (2009) Achievements of the RIANA and AWACSS EU projects immunosensors for the determination of pesticides, endocrine dismpting chemicals and pharmaceuticals. Hdb Env Chem 5J 33 6... 

Chlorophenoxy acid herbicides are also widely used to control broadleaf weeds and grass plants. Several immunoassays have been reported for 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T).246 247 Several immunosensors have been described using a transducing principle similar to the RIANA system already described in this chapter. Thus, Meusel et al.248 reported the use of monoclonal antibodies in a sensor chip to analyze river and lake water samples, obtaining detection limits of 0.1 ig L 1. Moreover, monoclonal antibodies, produced by Cuong et al.,249 were used in a dipstick immunoassay format to analyze pond water samples. When applied to the 2,4-D compound, this semiquantitative method yielded for an IC50 of 6 ug I. and an LOD of 0.5 pg L-1. 

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