Reversed phase system-grading

Ekgorg-Ott et al. (1997). An interesting trend was discovered when considering the relative amount of D-theanine present in the samples. The teas of the highest grades consistently contained the lowest amounts of D-theanine. The theanine achiral-chiral system configuration included a C18 column operated in the reverse-phase mode and a y-cyclodextrin CSP in the polar organic mode. 

While HPLC grade water is commercially available, I have found it to be expensive and to have limited shelf life. The best technique for purifying water seems to be to pass it through a bed of either reverse-phase packing material or of activated charcoal, as in a Milli-Q system. Even triple distillation tends to co-distill volatile impurities unless done using a fractionation apparatus. 

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