Reverse transcription, gene

Mikhitarian K, Reott S, Hoover L, et al. Enhanced detection of RNA from paraffin-embedded tissue using a panel of truncated gene-specific primers for reverse transcription. BioTechniques 2004 36 474 178. 

Ripe tomato fruits accumulate significant amounts of lycopene, but only trace amounts of xanthophylls. Dharmapuri and others (2002) overexpressed the lycopene (3-cyclase (b-Lcy) and (3-carotene hydroxylase (b-Chy) genes under the control of the fruit-specific Pds promoter, and transgene and protein expression was followed through semiquantitative reverse- transcription PCR, Western blotting, and enzyme assays. Fruits of the transformants showed a significant increase of (3-carotene, (3-cryptoxanthin, and zeaxanthin the carotenoid composition of leaves remained unaltered, and the transgenes and the phenotype were inherited in a dominant Mendelian fashion. 

Gettemy JM, Ma B, Alic M, Gold MH (1998) Reverse transcription PCR analysis of the regulation on the manganese peroxidases gene family. Appl Environ Microbiol 64(2) 569-574... 

Approaches to cloning DNA Genomic DNA Restriction endonucleases fragment DNA Total nuclear DNA cloned Genes contain introns cDNA Reverse transcription of mRNAs from cell Genes expressed cloned Genes have no introns... 

McGrath, S., Dolley, J.S.G. and Haylock, R.W., Quantification of Clostridium botulinum toxin gene expression by competitive reverse transcription PCR, Appl. Env. Microbiol., 66, 1423-1428, 2000. 

The vast majority of the smdies on antibiotic resistance in the environment have focussed on the survey of resistance genes. However, the mere detection of the antibiotic resistance genes may be insufficient to get a clear perspective of their function in the environment. If the role of antibiotic resistance genes in the environment is to be assessed, it is also important to determine the factors capable of triggering gene expression and to measure the expression levels [24]. Such an approach requires transcriptomic analyses supported, for instance, by reverse-transcription PCR or microarrays. 

Schonig K, Bujard H, Gossen M (2010) The power of reversibility regulating gene activities via tetracycline-controUed transcription. Methods Enzymol 477 429 53... 

Scherm, B., Palomba, M., Serra, D., Marcello, A., and Megheli, Q. (2005). Detection of transcripts of the aflatoxin genes aflD, aflO, and aflP by reverse transcription-polymerase chain reaction allows differentiation of alfatoxin-producing and non-producing isolates of Aspergillus flavus and Aspergillus parasiticus. Int. J. Food Microbiol. 98, 201-210. 

The protein truncation test is a way of testing large genes (e.g., NEl) for which an antibody is available. PTT can detect nonsense mutations that are peptide chain terminating. These show up, after reverse transcription/cell-free translation, as shorter-than-normal peptides in an electrophoretic gel (Eig. 3C). 

Yajima, T. Yagihashi, A. Kameshima, H. Furuya, D. Kobayashi, D. Hirata, K. Watanabe, N. Establishment of quantitative reverse transcription-polymerase chain reaction assays for human telomerase-associated genes. Clin. Chim. Acta 2000, 290(2), 117-127. 

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