Reverse phase chromatography defined

The term chemistry in interphases was first introduced in the field of reverse-phase chromatography [41], In 1995 Lindner et al. transferred the concept to the area of transition metal catalysis [42] and in a recent review the concept is explained in detail [43], The interphase is defined as a region within a system in which the stationary and a mobile component penetrate on a molecular level without the formation of a homogeneous mixture. In these regions the reactive centre on the stationary phase... 

Fatty acids have so far been analysed by CEC either as the free acids or as phenacyl- or methyl esters. Aqueous acetonitrile (50 mM) at pH 6 (9 1 v/v) was shown to be the optimal mobile phase [191]. It is generally known that in reversed-phase chromatography free fatty acids and fatty acid methyl esters separate according to the partition number, which is defined as the carbon number minus twice the number of double bonds. A double bond reduces the retention time by the equivalent... 

L)BPCis not the same as reversed phase chromatography (RPLC). RPLC can be defined as a form of liquid chromatography in which the mobile phase is more polar than the stationary phase, a situation that does not occur in GC. RPLC will be discussed extensively in section 3.2.2.I. 

The usual A solvent in reversed-phase chromatography is water or aqueous buffer. The strength of binary mixtures is not a well defined function of %B but depends on analyte and stationary phase properties. Nevertheless, it is possible to give numerical... 

The stationary phase in reversed-phase chromatography is poorly defined with a fluid structure, composition and volume that depends on the equilibrium mobile phase composition, the identity and bonding density of surface-restrained ligands, the number and type of accessible silanol groups, and temperature [108,109,240,243-... 

Triglycerides in vegetable oils are separated according to their equivalent carbon number by reversed-phase liquid chromatography and detected by differential refractometry. Quantitation is by area normalization. Elution order is determined by calculating the equivalent carbon numbers, ECNs, often defined (as we said before) as CN — 2n, where CN is the carbon number and n is the number of double bonds. To calculate ECNs more accurately, the origin of the double bond is taken into account ... 

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