Reusable gels

Reusable Gel Cassettes and Membrane-Mediated Sample Loading... 

REUSABLE GEL CASSETTES AND MEMBRANE-MEDIATED SAMPLE LOADING... 

The combination of pin printing and sol-gel processing techniques provides a simple method to rapidly fabricate reusable chemical sensor element into arrays that exhibit good analytical figures of merit. This methodology also provides a straightforward means to fabricate reusable sensor arrays for simultaneous multianalyte quantification. 

J. S. Yadav, S. Raghavendra, M. Satyanarayana, and E. Balanarsaiah, Phospho-molybdic acid supported on silica gel An efficient, mild and reusable catalyst for the chemoselective hydrolysis of acetonides, Synlett, 16 (2005) 2461-2464. 

Reusable Precast Gels Precast agarose gels are now available that may be recycled. After a run, the DNA samples on the gel are removed by reversing the direction of the electric field. The gels are then reloaded with new samples and reused. 

Equipment essential for electrophoresis includes power sources, gel molds, sample grinding trays, electrode buffer trays, reusable ice packs (or a 4° cold room), a gel slicer, staining trays, assorted glassware, variable temperature incubators), hot/stir plates, and a pH meter. Details on equipping a laboratory for electrophoresis are given elsewhere.12,17... 

Place a gel, loaded with samples, on a block of reusable ice between two reservoirs containing electrode buffer. Connect the reservoirs to the power supply using electrical leads fitted with male banana plugs. Be sure that the entire bottom of the gel mold is in contact with the ice so that cooling is even. Orient the gel so that the wicks are closest to the negative pole (black or cathode). Use cellulose sponges or Handiwipes (available in most gro-... 

Angeletti E, Canepa C, Martinetti G, Venturello P (1989) Amino groups immobilized on silica gel An efficient and reusable heterogeneous catalyst for the knoevenagel condensation. J Chem Soc Perkin Trans 1 105... 

ILs have been used for immobilization of enzymes. Horseradish peroxidase (HRP) activity is evaluated in a [BMIM][BF ] sol-gel matrix [26]. Higher activity in [BMIM][PFg] is also observed when lipase from an unspecified Pseudomonas (PsL) is immobilized on methacryloxypropyl-modified mesoporous silica [27]. Similarly, IL-coated enzyme was found to completely retain the activity and easily reusable [28]. 

Silica-gel supported polyphosphoric acid (PPA/Si02) has been developed as a reusable catalyst-oxathio- and dithio- acetals were synthesized by using PPA/Si02. The recovered catalyst can be reused nine successive times, after drying each time at 100 °C for 2 h under reduced pressure [60],... 

Coupling reactions. Various coupling reactions involving nonpolar substrates may be carried out with hydrophobicized silica sol-gel matrix in which Pd(OAc)2 is trapped. The substrates form microemulsions in water with SDS and ROH. The catalyst is leach-proof and reusable. ... 

Silica Gel. Toishi (21) found that internal case humidity values could be controlled chemically with preconditioned silica gel, which can absorb over 30% moisture by weight (13). When preconditioned to 55% RH and placed inside a display case, the gel not only reduced humidity variations, but also buffered the small changes in RH 21, 22). In fact, not only was silica gel a good reusable buffer, but it also allowed for a specific RH to be set within the case. However, its use can be financially prohibitive. Large quantities are needed to properly regulate display case conditions, and reconditioning this bulk can be labor-intensive. 

As mentioned earlier, the way of immobilizing enzymes plays a key role in enzyme stability, solubility and reusability. In addition, mass transport limitations can affect reaction rates drastically. Liu et al. immobilized horseradish peroxidase (HRP) in a [BMIM][Bp4] based sol-gd matrix. Sol-gel-derived silica glasses are very popular for encapsulation of biomolecules. Compared to results in the conventional... 

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