Restriction enzymes polymorphisms

RFLP). Variation between individuals in DNA fragment sizes cut by specific restriction enzymes polymorphic sequences that result in RFLPs are used as markers on both physical maps and genetic... 

The detection of restriction fi agment length polymorphisms (RFLPs) facilitates prenatal detection of hereditary disorders such as sickle cell trait, beta-thalassemia, infant phenylketonuria, and Huntington s disease. Detection of RFLPs involves cleavage of double-stranded DNA by restriction endonucleases, which can detect subtle alterations in DNA that affect their recognized sites. Chapter 40 provides further details concerning the use of PCR and restriction enzymes for diagnosis. 

Restriction enzymes, sequence-dependent cleavage of DNA by, 12 497—498 Restriction fragment linked polymorphism (RFLP), 12 500 procedure, 12 103-104 Restriction sites, as genetic markers, 12 500... 

Studies by David Botstein and others found that when a restrictive enzyme is applied to DNA from different individuals, the resulting sets of fragments sometimes differ markedly from one person ro the nexr Such variations in DNA are called restriction fragment length polymorphisms, or RFLPs, and they are extremely useful in genetic studies. 

Whereas DNA polymorphisms should be recognizable by sequence differences, it is usually more convenient to detect these polymorphisms by differences in the size of DNA fragments obtained with restriction enzymes. Differences observed in this way are called restriction fragment length polymorphisms (RFLPs). 

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